Inhibition of ceramide synthesis reverses endothelial dysfunction and atherosclerosis in streptozotocin-induced diabetic rats
| Autor: | Li Chun, Li Niansheng, Chen Benmei, Wang Aimin, Zhou Junlin, Lei Min-xiang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Ceramide Endothelium Endocrinology Diabetes and Metabolism Blotting Western Ceramides Nitric Oxide Mass Spectrometry Diabetes Mellitus Experimental Fatty Acids Monounsaturated Rats Sprague-Dawley chemistry.chemical_compound Endocrinology Microscopy Electron Transmission Enos Diabetes mellitus Internal medicine Myriocin Internal Medicine medicine Animals Endothelial dysfunction biology Cholesterol business.industry General Medicine Atherosclerosis Streptozotocin medicine.disease biology.organism_classification Immunohistochemistry Rats medicine.anatomical_structure chemistry Endothelium Vascular business Chromatography Liquid medicine.drug |
| Zdroj: | Diabetes Research and Clinical Practice. 93:77-85 |
| ISSN: | 0168-8227 |
| Popis: | To explore the effect of myriocin on EDVD and atherosclerosis in diabetic rats.Rats were fed with a high-fat/high-sucrose/high-cholesterol diet (20% sucrose, 10% animal oil, 1.0% bile salt and 2.5% cholesterol) (hereinafter defined as diabetic groups) or Purina Rodent Chow (NC group), the former was intervened with low dose streptozotocin (30 mg/kg) after feeding 1 month to make diabetic model. The NC group was intervened with citrate buffer and the diabetic rats were intervened with myriocin (0.3 mg/kg Qod) (MTD group) or just solvent (DC group) for 14 weeks. The EDVD, thickness of fatty deposition under endothelium, ceramide, PI3K/PKB/eNOS, NO and other vital parameters were measured after the rats sacrificed.In DC group, the ceramide contents in serum and aorta increased, the EDVD was impaired, the fatty deposition under endothelium increased, and the phosphorylation of PI3K/PKB/eNOS and NO release decreased all compared with the NC group (P0.05). Compared with the DC group, the ceramide contents in MTD group decreased, the EDVD ameliorated, the fatty deposition diminished, and PI3K/PKB/eNOS phosphorylation and NO release (P0.05) increased.After treated with myriocin, the EDVD in diabetic rats has been improved by increasing PI3K/PKB/eNOS phosphorylation and NO release, and meanwhile the atherosclerosis has reversed. |
| Databáze: | OpenAIRE |
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