Expression analyses of three members of the AtPHO1 family reveal differential interactions between signaling pathways involved in phosphate deficiency and the responses to auxin, cytokinin, and abscisic acid

Autor: Cécile Ribot, Yong Wang, Yves Poirier
Přispěvatelé: Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), School of Computer Science and Engineering [Guilin University of Electronic Technology], Guilin University of Electronic Technology, Département de Biologie Moléculaire Végétale, Biophore, Université de Lausanne, Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)
Jazyk: angličtina
Rok vydání: 2008
Předmět:
0106 biological sciences
Sucrose
Cytokinins
Phosphatase
Arabidopsis
Plant Science
Biology
Plant Roots
01 natural sciences
Phosphates
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
03 medical and health sciences
chemistry.chemical_compound
Gene Expression Regulation
Plant
Xylem
Auxin
Genetics
Arabidopsis thaliana
Gene family
Abscisic Acid/pharmacology
Arabidopsis/drug effects
Arabidopsis/genetics
Arabidopsis Proteins/genetics
Arabidopsis Proteins/metabolism
Blotting
Northern
Cytokinins/pharmacology
Gene Expression Regulation
Plant/drug effects
Indoleacetic Acids/pharmacology
Phosphates/metabolism
Plant Roots/drug effects
Plant Roots/genetics
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction/drug effects
Signal Transduction/genetics
Sucrose/pharmacology
Xylem/drug effects
Xylem/genetics
Abscisic acid
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
chemistry.chemical_classification
Regulation of gene expression
0303 health sciences
Indoleacetic Acids
Arabidopsis Proteins
fungi
food and beverages
biology.organism_classification
chemistry
Biochemistry
Cytokinin
Abscisic Acid
Signal Transduction
010606 plant biology & botany
Zdroj: Planta
Planta, Springer Verlag, 2008, 227 (5), pp.1025-1036. ⟨10.1007/s00425-007-0677-x⟩
Planta, vol. 227, no. 5, pp. 1025-1036
ISSN: 0032-0935
1432-2048
DOI: 10.1007/s00425-007-0677-x⟩
Popis: The PHO1 protein is involved in loading inorganic phosphate (Pi) to the root xylem. Ten genes homologous to AtPHO1 are present in the Arabidopsis thaliana (L.) Heyn genome. From this gene family, transcript levels of only AtPHO1, AtPHO1;H1 and AtPHO1;H10 were increased by Pi-deficiency. While the up-regulation of AtPHO1;H1 and AtPHO1;H10 by Pi deficiency followed the same rapid kinetics and was dependent on the PHR1 transcription factor, phosphite only strongly suppressed the expression of AtPHO1;H1 and had a minor effect on AtPHO1;H10. Addition of sucrose was found to increase transcript levels of both AtPHO1 and AtPHO1;H1 in Pi-sufficient or Pi-deficient plants, but to suppress AtPHO1:H10 under the same conditions. Treatments of plants with auxin or cytokinin had contrasting effect depending on the gene and on the Pi status of the plants. Thus, while both hormones down-regulated expression of AtPHO1 independently of the plant Pi status, auxin and cytokinin up-regulated AtPHO1;H1 and AtPHO1;H10 expression in Pi-sufficient plants and down-regulated expression in Pi-deficient plants. Treatments with abscisic acid inhibited AtPHO1 and AtPHO1;H1 expression in both Pi-sufficient and Pi-deficient plants, but increased AtPHO1;H10 expression under the same conditions. The inhibition of expression by abscisic acid of AtPHO1 and AtPHO1;H1, and of the Pi-starvation responsive genes AtPHT1;1 and AtIPS1, was dependant on the ABI1 type 2C protein phosphatase. These results reveal that various levels of cross talk between the signal transduction pathways to Pi, sucrose and phytohormones are involved in the regulation of expression of the three AtPHO1 homologues.
Databáze: OpenAIRE
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