Multilocus sequence typing analysis reveals that Cryptococcus neoformans var. neoformans is a recombinant population

Autor: Ashok Varma, Kathrin Tintelnot, Patricia Escandón, Tomoe Ichikawa, Aristea Velegraki, Volker Rickerts, Alberto Zani, Reiko Ikeda, Ilka McCormick, Anne-Lise Bienvenu, Sevim Akcaglar, Massimo Cogliati, Anna Maria Tortorano, Okan Töre, Marie Desnos-Ollivier, Shawn R. Lockhart
Přispěvatelé: Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı., Töre, Okan, Akçağlar, Sevim, Università degli Studi di Milano = University of Milan (UNIMI), Robert Koch Institute [Berlin] (RKI), Centre National de Référence des Mycoses invasives et antifongiques - Mycologie moléculaire (CNRMA), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), National and Kapodistrian University of Athens (NKUA), Instituto Nacional de Salud [Bogota], Meiji Pharmaceutical University, Hospices Civils de Lyon (HCL), Université de Lyon, Uludağ Üniversitesi = Uludag University, Centers for Disease Control and Prevention [Atlanta] (CDC), Centers for Disease Control and Prevention, National Institutes of Health [Bethesda] (NIH), We thank Prof. F. Dromer and Prof. J. Kwon-Chung for providing some of the isolates and for her suggestions to improve this study, and Dr. T. Boekhout and Dr. B. Theelen for sequencing support., Università degli Studi di Milano [Milano] (UNIMI), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Serotype
C neoformans var grubii
Serotypes
Denmark
Cryptococcus Gattii
Flucytosine
Cryptococcus Neoformans
Cryptococcus
Molecular typing
Population structure
Turkey (republic)
law.invention
MESH: Genotype
Species complex
Belgium
Japan
law
Germany
Genotype
MESH: Genetic Variation
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Phylogeny
Priority journal
education.field_of_study
Phylogenetic tree
biology
Greece
MESH: Asia
Genetics & heredity
C. neoformans var. neoformans
Correlation analysis
MESH: Cryptococcus neoformans
Gene linkage disequilibrium
Cryptococcosis
Thailand
Classification
3. Good health
Europe
Phylogeography
MESH: Multilocus Sequence Typing
Italy
MESH: Phylogeography
Molecular epidemiology
Recombinant DNA
MESH: Recombination
Genetic
Microbiological examination
France
MLST
Asia
030106 microbiology
Population
C neoformans var neoformans
Multilocus sequence typing
MESH: Cryptococcosis
Mycology
Recombination
genetic
Colombia
Microbiology
Mating-type
Article
03 medical and health sciences
C. neoformans var. grubii
C neoformans var. grubii
MESH: Mycological Typing Techniques
Genetics
MESH: Americas
Cutaneous cryptococcosis
Genetic variation
Western hemisphere
education
Cryptococcus neoformans
Genetic recombination
Mycological typing techniques
biology.organism_classification
Nonhuman
Recombination
United States
Gattii
030104 developmental biology
Isolation and purification
Geographic origin
Fungus isolation
Genetic variability
MESH: Europe
Americas
Varietas
Zdroj: Fungal Genetics and Biology
Fungal Genetics and Biology, 2016, 87, pp.22-29. ⟨10.1016/j.fgb.2016.01.003⟩
Fungal Genetics and Biology, Elsevier, 2016, 87, pp.22-29. ⟨10.1016/j.fgb.2016.01.003⟩
ISSN: 1087-1845
1096-0937
DOI: 10.1016/j.fgb.2016.01.003⟩
Popis: Cryptococcus neoformans var. neoformans (serotype D) represents about 30% of the clinical isolates in Europe and is present less frequently in the other continents. It is the prevalent etiological agent in primary cutaneous cryptococcosis as well as in cryptococcal skin lesions of disseminated cryptococcosis. Very little is known about the genotypic diversity of this Cryptococcus subtype. The aim of this study was to investigate the genotypic diversity among a set of clinical and environmental C. neoformans var. neoformans isolates and to evaluate the relationship between genotypes, geographical origin and clinical manifestations. A total of 83 globally collected C neoformans var. neoformans isolates from Italy, Germany, France, Belgium, Denmark, Greece, Turkey, Thailand, Japan, Colombia, and the USA, recovered from different sources (primary and secondary cutaneous cryptococcosis, disseminated cryptococcosis, the environment, and animals), were included in the study. All isolates were confirmed to belong to genotype VNIV by molecular typing and they were further investigated by MLST analysis. Maximum likelihood phylogenetic as well as network analysis strongly suggested the existence of a recombinant rather than a clonal population structure. Geographical origin and source of isolation were not correlated with a specific MLST genotype. The comparison with a set of outgroup C neoformans var. grubii isolates provided clear evidence that the two varieties have different population structures. United States Department of Health & Human Services - Z99 AI999999 - P41 RR001646 National Institutes of Health (NIH) - USA NIH National Center for Research Resources (NCRR)
Databáze: OpenAIRE
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