A Novel Role for DNA Photolyase: Binding to DNA Damaged by Drugs Is Associated with Enhanced Cytotoxicity in Saccharomyces cerevisiae
| Autor: | Brian J. Feldman, Gilbert Chu, M E Fox |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Methylnitronitrosoguanidine
Saccharomyces cerevisiae Proteins DNA damage 4-Nitroquinoline 1-oxide Saccharomyces cerevisiae Pyrimidine dimer Biology Fungal Proteins chemistry.chemical_compound Mechlorethamine DNA Fungal Photolyase Molecular Biology Endodeoxyribonucleases Dose-Response Relationship Drug Cell Biology DNA photolyase biology.organism_classification Molecular biology 4-Nitroquinoline-1-oxide DNA-Binding Proteins chemistry Biochemistry Pyrimidine Dimers Cisplatin Deoxyribodipyrimidine Photo-Lyase DNA DNA Damage Research Article Nucleotide excision repair |
| Zdroj: | Molecular and Cellular Biology. 14:8071-8077 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.14.12.8071-8077.1994 |
| Popis: | DNA photolyase binds to and repairs cyclobutane pyrimidine dimers induced by UV radiation. Here we demonstrate that in the yeast Saccharomyces cerevisiae, photolyase also binds to DNA damaged by the anticancer drugs cis-diamminedichloroplatinum (cis-DDP) and nitrogen mustard (HN2) and by the alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Surprisingly, mutations in photolyase were associated with resistance of yeast cells to cis-DDP, MNNG, 4-nitroquinoline oxide (4NQO), and HN2. Transformation of yeast photolyase mutants with the photolyase gene increased sensitivity to these agents. Thus, while the binding of photolyase to DNA damaged by UV radiation aids survival of the cell, binding to DNA damaged by other agents may interfere with cell survival, perhaps by making the lesions inaccessible to the nucleotide excision repair system. |
| Databáze: | OpenAIRE |
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