Rapid Point-of-Care Assay by SERS Detection of SARS-CoV-2 Virus and Its Variants
| Autor: | Peng-Cheng Guan, Hong Zhang, Zhi-Yong Li, Shan-Shan Xu, Miao Sun, Xian-Min Tian, Zhan Ma, Jia-Sheng Lin, Man-Man Gu, Huan Wen, Fan-Li Zhang, Yue-Jiao Zhang, Guang-Jun Yu, Chaoyong Yang, Zhan-Xiang Wang, Yanling Song, Jian-Feng Li |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Analytical Chemistry. 94:17795-17802 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Addressing the spread of coronavirus disease 2019 (COVID-19) has highlighted the need for rapid, accurate, and low-cost diagnostic methods that detect specific antigens for SARS-CoV-2 infection. Tests for COVID-19 are based on reverse transcription PCR (RT-PCR), which requires laboratory services and is time-consuming. Here, by targeting the SARS-CoV-2 spike protein, we present a point-of-care SERS detection platform that specifically detects SARS-CoV-2 antigen in one step by captureing substrates and detection probes based on aptamer-specific recognition. Using the pseudovirus, without any pretreatment, the SARS-CoV-2 virus and its variants were detected by a handheld Raman spectrometer within 5 min. The limit of detection (LoD) for the pseudovirus was 124 TU μL |
| Databáze: | OpenAIRE |
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