Toxic effect of acetamiprid on Rana ridibunda sciatic nerve (electrophysiological and histopathological potential)
| Autor: | Şakir Necat Yılmaz, Yusuf Çamlica, Salih Cüfer Bediz, Ulku Comelekoglu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Insecticides
medicine.medical_specialty Health Toxicology and Mutagenesis Action Potentials In Vitro Techniques 010501 environmental sciences Pharmacology Biology Toxicology 01 natural sciences Acetamiprid Neonicotinoids 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Microscopy Electron Transmission medicine Animals Rana ridibunda 0105 earth and related environmental sciences Chemical Health and Safety Dose-Response Relationship Drug Public Health Environmental and Occupational Health Neonicotinoid insecticide General Medicine Sciatic Nerve Electrophysiology chemistry Histopathology Sciatic nerve 030217 neurology & neurosurgery |
| Zdroj: | Drug and Chemical Toxicology. 42:264-269 |
| ISSN: | 1525-6014 0148-0545 |
| Popis: | In this study, the effects of a neonicotinoid insecticide acetamiprid on the sciatic nerve of Rana ridibunda were investigated by using electrophysiological and histological methods. A total of 35 preparations of sciatic nerve isolated from 35 frogs (Nervus ischiadicus) were used in the experiments. Experiments were designed as four different dose groups (n = 8 per group). Acetamiprid solutions of 1 (group 1), 10 (group 2), 100 (group 3), and 1000 µM (group 4) were applied to the nerves in dose groups. In each group, action potentials were recorded before application of acetamiprid which served as control data. The extracellular action potentials were recorded for each group of 30th, 60th, 90th and 120th min of application time. Action potential amplitude and area were measured from recordings. Histological evaluation was performed by transmission electron microscopy. In electrophysiological examination, all doses in which acetamiprid applied have shown the effect from the 30th min and suppressed the sciatic nerve action potential. Acetamiprid significantly reduced the amplitude at the rate of 78-96% and the area at the rate of 79-98% (p 0.05). In electron microscopic examination, the control nerves were in normal appearance. Disorganization, irregularity, dense ovoid body formation, fragmentation of the myelin sheath, and loss on some axoplasm of the nerves in the dose group have been observed. Our findings showed that acetamiprid can cause neuropathic changes in sciatic nerve at all applied doses. These results indicate that acetamiprid as other insecticides can have harmful effects on non-target organisms. |
| Databáze: | OpenAIRE |
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