Arabidopsis AGDP1 links H3K9me2 to DNA methylation in heterochromatin
| Autor: | Kai Tang, P. Zhu, W.A. Tao, Heng Zhang, Yuhui Jiang, H. Wan, F. Wu, Ray A. Bressan, Xin-Jian He, Xingang Wang, Jian-Kang Zhu, Zhusheng Yang, Xiaofan Du, Cui-Jun Zhang, Jiamu Du, Li Pan, Jianying Luo |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Heterochromatin Science Arabidopsis General Physics and Astronomy 01 natural sciences DNA-binding protein General Biochemistry Genetics and Molecular Biology Article Protein Structure Secondary 03 medical and health sciences Epigenetics Amino Acid Sequence Gene Silencing lcsh:Science Regulation of gene expression Multidisciplinary biology Chemistry Arabidopsis Proteins Lysine General Chemistry Methylation DNA Methylation Chromatin Cell biology 030104 developmental biology Histone Genetic Loci DNA methylation biology.protein DNA Transposable Elements lcsh:Q Peptides 010606 plant biology & botany Protein Binding |
| Zdroj: | Nature Communications, Vol 9, Iss 1, Pp 1-14 (2018) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Heterochromatin is a tightly packed form of chromatin that is associated with DNA methylation and histone 3 lysine 9 methylation (H3K9me). Here, we identify an H3K9me2-binding protein, Agenet domain (AGD)-containing p1 (AGDP1), in Arabidopsis thaliana. Here we find that AGDP1 can specifically recognize the H3K9me2 mark by its three pairs of tandem AGDs. We determine the crystal structure of the Agenet domain 1 and 2 cassette (AGD12) of Raphanus sativus AGDP1 in complex with an H3K9me2 peptide. In the complex, the histone peptide adopts a unique helical conformation. AGD12 specifically recognizes the H3K4me0 and H3K9me2 marks by hydrogen bonding and hydrophobic interactions. In addition, we find that AGDP1 is required for transcriptional silencing, non-CG DNA methylation, and H3K9 dimethylation at some loci. ChIP-seq data show that AGDP1 preferentially occupies long transposons and is associated with heterochromatin marks. Our findings suggest that, as a heterochromatin-binding protein, AGDP1 links H3K9me2 to DNA methylation in heterochromatin regions. DNA methylation and H3K9 dimethylation are two linked epigenetic marks of silenced chromatin in plants that depend on the activity of CMT3/2 and SUVH4/5/6. Here the authors identify AGDP1 as an H3K9me2-binding protein required for heterochromatic non-CG DNA methylation, H3K9 dimethylation, and transcriptional silencing. |
| Databáze: | OpenAIRE |
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