Effect of root surface treatment with denusomab after delayed tooth replantation

Autor: Daniele Lucca Longo, Maria Bernadete Sasso Stuani, Raquel Assed Bezerra da Silva, Paulo Nelson-Filho, Léa Assed Bezerra da Silva, Carolina Maschietto Pucinelli, Heloisa Aparecida Orsini Vieira, Alexandra Mussolino de Queiroz, Francisco Wanderley Garcia Paula-Silva
Rok vydání: 2020
Předmět:
Zdroj: Clinical Oral Investigations. 25:1255-1264
ISSN: 1436-3771
1432-6981
DOI: 10.1007/s00784-020-03430-1
Popis: To evaluate denosumab, a human monoclonal antibody that mimics the effects of osteoprotegerin in bone metabolism, as a topical treatment of root surface to be used prior to delayed tooth replantation. Thirty-six rats’ right incisors were used. Teeth were extracted and divided into: delayed replantation without root surface treatment (control); delayed replantation with root surface treatment with denosumab 60 mg/mL and 30 mg/mL, respectively, for 10 min both experimentals groups. After that, the root canals were filled with calcium hydroxide and replanted. After 15 and 60 days, the animals were euthanized, and the samples were collected and processed for microscopic analysis. Histological sections were performed, and stained with HE to describe the dental characteristics, measure ankylosis, replacement resorption, and dental resorption by conventional microscopy. Also, was performed Brown & Brenn staining and immunohistochemistry for RANKL, OPG, and periostin. Denosumab 60 mg/mL reducted ankylosis (p
Databáze: OpenAIRE
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