Nup358 interacts with APC and plays a role in cell polarization
| Autor: | Jomon Joseph, Prayag Murawala, Aparna Salunke, Pankhuri Vyas, Mukesh Mani Tripathi |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
biology
Adenomatous polyposis coli Adenomatous Polyposis Coli Protein Cell Polarity Cell migration Cell Biology Microtubules Cell Line Protein Structure Tertiary Cell biology Nuclear Pore Complex Proteins Protein Transport Cell Movement Microtubule RNA interference Cell cortex biology.protein Animals Humans Ectopic expression Nucleoporin RANBP2 Molecular Chaperones Protein Binding |
| Zdroj: | Journal of Cell Science. 122:3113-3122 |
| ISSN: | 1477-9137 0021-9533 |
| Popis: | Asymmetric localization of adenomatous polyposis coli (APC) to the ends of a subset of microtubules located in the leading edges is essential for the establishment of front-rear polarity during cell migration. APC is known to associate with microtubules in three ways: through interaction with the plus-end tracking protein EB1, direct binding through a C-terminal basic region, and through interaction with the plus-end motor kinesin-2. Here we report that the middle region of APC has a previously unidentified microtubule plus-end-targeting function, suggesting an additional microtubule-binding mode for APC. Through the same region, APC interacts with Nup358 (also called RanBP2), a microtubule-binding nucleoporin. Ectopic expression of the middle region of APC is sufficient to recruit endogenous Nup358 to the plus ends of microtubules. Furthermore, our results indicate that Nup358 cooperates with kinesin-2 to regulate the localization of APC to the cell cortex through a nuclear-transport-independent mechanism. Using RNA interference and a scratch-induced wound-healing assay we demonstrate that Nup358 functions in polarized cell migration. These results reveal a more active role for structural nucleoporins in regulating fundamental cellular processes than previously anticipated. |
| Databáze: | OpenAIRE |
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