Detection of the DeltaF508 mutation in the CFTR gene by means of time-resolved fluorescence methods
| Autor: | D Turner, G. Löber, S. Winter, S. Kirschstein |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Boron Compounds
Base Sequence Oligonucleotide Point mutation Biophysics Cystic Fibrosis Transmembrane Conductance Regulator Biology Fluorescence Molecular biology Polymerase Chain Reaction Cystic fibrosis transmembrane conductance regulator law.invention Spectrometry Fluorescence law Mutation (genetic algorithm) Electrochemistry biology.protein Nucleic acid Point Mutation Physical and Theoretical Chemistry Gene Polymerase chain reaction In Situ Hybridization Fluorescence DNA Primers Fluorescent Dyes |
| Zdroj: | Bioelectrochemistry and bioenergetics (Lausanne, Switzerland). 48(2) |
| ISSN: | 0302-4598 |
| Popis: | A rapid recognition in the base sequence of nucleic acids is an important prerequisite toward the diagnosis of genetic diseases and their carrier states. We have developed a hybridisation method in which a fluorescently labeled oligonucleotide is used to detect point mutations in a target by a simple fluorescence lifetime analysis of the emission of the fluorescent label. We applied this method to detect the deltaF508 mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene in a model system and with biologically derived PCR product and discuss the potential generality of this method. |
| Databáze: | OpenAIRE |
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