Analysis of the frequency of type 2 innate lymphoid cells and regulatory T cells in abortion-prone mice
| Autor: | Amin Derakhshanfar, Mahsa Sadat Hosseini, Mojgan Akbarzadeh-Jahromi, Mohammad Ali-Hassanzadeh, Moslem Ahmadi, Behrouz Gharesi-Fard, Maryam Zare |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Immunology Uterus Abortion T-Lymphocytes Regulatory Andrology Mice 03 medical and health sciences Th2 Cells 0302 clinical medicine Immune system Immunophenotyping Pregnancy Decidua medicine Animals Immunology and Allergy business.industry Innate lymphoid cell medicine.disease Immunity Innate Abortion Spontaneous 030104 developmental biology medicine.anatomical_structure Mice Inbred DBA Mice Inbred CBA Female Complication business 030215 immunology Hormone |
| Zdroj: | Immunology Letters. 220:1-10 |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/j.imlet.2020.01.002 |
| Popis: | Recurrent spontaneous abortion (RSA) is the most common pregnancy related complication, affecting 1-5 % of pregnancies. Despite hormonal, genetic and anatomical factors that result in abortion, impairment of immune response at the feto-maternal interface during the first trimester of pregnancy is also one of the main causes of RSA. In the present study, we evaluated the frequency of blood and uterine group 2 innate lymphoid cells (ILC2s), their subsets and regulatory T cells (Tregs) in CBA/J × DBA/2 J as an abortion-prone model compared to normal pregnant (NP) mice using immunophenotyping. Results indicated that the percentages of ILC2s were significantly decreased in the AP group compared to the NP group at mid-gestation (P ≤ 0.01). Moreover, the percentages of both blood and uterine nILC2s were increased in NP mice at mid-gestation (P ≤ 0.01, and P ≤ 0.05, respectively), while iILC2s significantly increased in AP mice at mid-gestation (P ≤ 0.01, and P ≤ 0.05, respectively). Tregs were reduced in AP mice at both early and mid-gestation stages (P ≤ 0.01). Overall, our findings suggest that the changes in blood and uterine ILC2s might be associated with abortion in mice. |
| Databáze: | OpenAIRE |
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