67 laminin receptor promotes the malignant potential of tumour cells up-regulating lysyl oxidase-like 2 expression in cholangiocarcinoma
| Autor: | Peng Jiang, Jing Xu, Qiang He, Shuguang Wang, Yang Gao, Tianyu Li, Dajing Li, De-chun Wang, Zipei Liu, Xiaowu Li, Feng Tian |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Ribosomal Proteins Pathology medicine.medical_specialty Mice Nude Lysyl oxidase digestive system Metastasis Cholangiocarcinoma Receptors Laminin Small hairpin RNA Mice Cell Movement Cell Line Tumor Biomarkers Tumor medicine Animals Humans Neoplasm Invasiveness neoplasms Aged Gene knockdown Hepatology LOXL2 business.industry Gastroenterology Cell Differentiation Transfection Middle Aged Prognosis medicine.disease digestive system diseases Up-Regulation Survival Rate 67 kDa Laminin Receptor Bile Ducts Intrahepatic Bile Duct Neoplasms Gene Knockdown Techniques Lymphatic Metastasis Immunohistochemistry Female Amino Acid Oxidoreductases business |
| Zdroj: | Digestive and Liver Disease. 46:750-757 |
| ISSN: | 1590-8658 |
| DOI: | 10.1016/j.dld.2014.03.017 |
| Popis: | Background 67 laminin receptor (67LR) plays an important role in the invasion and metastasis of cholangiocarcinoma, but its mechanism remains unclear. Aims We investigated the clinical significance of 67LR and its relation to lysyl oxidase-like 2 (LOXL2) in 67LR-mediated invasion and metastasis in cholangiocarcinoma. Methods The clinical significance of 67LR and LOXL2 expression and the prognosis of patients were investigated in 73 cancerous and 32 paracancerous tissues by immunohistochemistry. The impact of LOXL2 on invasion, metastasis and 67LR expression was evaluated in cholangiocarcinoma cells by shRNA or expressed-plasmid transfection. Results Expression of 67LR was recognized in 35.62% cholangiocarcinoma tissue, and none in paracancerous tissues. LOXL2 was positively correlated with expression of 67LR. Expression of 67LR or LOXL2 in cholangiocarcinomas was significantly associated with lymph node metastasis, differentiation and poor overall survival. Cox analysis showed that 67LR can act as an independent prognostic biomarker of prognosis in cholangiocarcinoma patients. Expression of LOXL2 decreased by knockdown of 67LR and increased by overexpression of 67LR in cholangiocarcinoma cells. Knockdown of LOXL2 reduced invasion and metastasis in vitro and in vivo. Conclusion 67LR may regulate the expression of LOXL2 to promote invasion and metastasis in cholangiocarcinoma cells. It could be used as an independent prognostic marker in cholangiocarcinoma patients. |
| Databáze: | OpenAIRE |
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