Use of the method of homologous recombination for site-directed mutagenesis of the drosophila-d4 gene of Drosophila melanogaster

Autor: Maxim, Lazarev, Laskina Tatyana
Jazyk: ruština
Rok vydání: 2021
Předmět:
DOI: 10.5281/zenodo.6475527
Popis: This work is devoted to the analysis of translocation from the genetic construct of the DNA fragment of the mutant dd4 gene in the Drosophila melanogaster genome using the yeast FRT-FLP recombination system and I-SceI restriction. Achievement of the goal was solved by setting three tasks: 1) Chromosomal mapping of the inactivating gene construct p {whs pW25 dd4-exc}; 2) Genetic control of the movement of a destabilized construct containing a non-functional copy of the dd4 gene; 3) Molecular analysis of DNA of lines subjected to site-directed mutagenesis.
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Databáze: OpenAIRE
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