Antibacterial and Cytotoxic Activity of Compounds Isolated fromFlourensia oolepis
| Autor: | Mariana Belén Joray, Sara M. Palacios, María Laura González, José Luis Bocco, Georgina Natalia Diaz Napal, Lucas D Trucco, María Cecilia Carpinella |
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| Rok vydání: | 2015 |
| Předmět: |
Chalcone
PLANTAS NATIVAS Article Subject genetic structures Otras Ciencias Biológicas Pharmacology behavioral disciplines and activities Ciencias Biológicas purl.org/becyt/ford/1 [https] chemistry.chemical_compound Minimum inhibitory concentration Cytotoxic T cell Medicine MTT assay purl.org/becyt/ford/1.6 [https] Cytotoxicity Pinocembrin business.industry lcsh:Other systems of medicine lcsh:RZ201-999 ANTIBACTERIANOS NATURALES nervous system Complementary and alternative medicine chemistry Cell culture CITOTOXICOS NATURALES business CIENCIAS NATURALES Y EXACTAS psychological phenomena and processes Isoliquiritigenin Research Article |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Evidence-based Complementary and Alternative Medicine : eCAM Evidence-Based Complementary and Alternative Medicine, Vol 2015 (2015) |
| ISSN: | 1741-4288 1741-427X |
| Popis: | The antibacterial and cytotoxic effects of metabolites isolated from an antibacterial extract of Flourensia oolepis were evaluated. Bioguided fractionation led to five flavonoids, identified as 2′,4′-dihydroxychalcone (1), isoliquiritigenin (2), pinocembrin (3), 7-hydroxyflavanone (4), and 7,4′-dihydroxy-3′-methoxyflavanone (5). Compound 1 showed the highest antibacterial effect, with minimum inhibitory concentration (MIC) values ranging from 31 to 62 and 62 to 250 g/mL, against Gram-positive and Gram-negative bacteria, respectively. On further assays, the cytotoxic effect of compounds 1-5 was determined by MTT assay on acute lymphoblastic leukemia (ALL) and chronic myeloid leukemia (CML) cell lines including their multidrug resistant (MDR) phenotypes. Compound 1 induced a remarkable cytotoxic activity toward ALL cells (IC50 = 6.6-9.9 M) and a lower effect against CML cells (IC50 = 27.5-30.0 M). Flow cytometry was used to analyze cell cycle distribution and cell death by PI-labeled cells and by Annexin V/PI staining, respectively. Upon treatment, 1 induced cell cycle arrest in the G2/M phase accompanied by a strong induction of apoptosis. These results describe for the first time the antibacterial metabolites of F. oolepis extract, with 1 being the most effective. This chalcone also emerges as a selective cytotoxic agent against sensitive and resistant leukemic cells, highlighting its potential as a lead compound. Fil: Joray, Mariana Belén. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Trucco, Lucas Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: González, María Laura. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Diaz Napal, Georgina Natalia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Palacios, Sara Maria. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bocco, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Carpinella, Maria Cecilia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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