Gene expression profiling of cutaneous wound healing
Autor: | Ping Jin, Kina Smith, Francesco M. Marincola, Kavita Deonarine, Monica C. Panelli, Ena Wang, Mitchell E Stashower, Herbert B. Slade, Christopher Norwood, David F. Stroncek |
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Jazyk: | angličtina |
Rok vydání: | 2007 |
Předmět: |
Pathology
medicine.medical_specialty Angiogenesis Administration Topical Biopsy lcsh:Medicine Imiquimod Inflammation Biology General Biochemistry Genetics and Molecular Biology Placebos medicine Cluster Analysis Humans Basal cell carcinoma Skin Regulation of gene expression Wound Healing medicine.diagnostic_test Research Gene Expression Profiling Macrophages lcsh:R General Medicine medicine.disease Gene expression profiling Gene Expression Regulation Neoplastic Aminoquinolines medicine.symptom Wound healing Biomarkers medicine.drug |
Zdroj: | Journal of Translational Medicine Journal of Translational Medicine, Vol 5, Iss 1, p 11 (2007) |
ISSN: | 1479-5876 |
Popis: | Background Although the sequence of events leading to wound repair has been described at the cellular and, to a limited extent, at the protein level this process has yet to be fully elucidated. Genome wide transcriptional analysis tools promise to further define the global picture of this complex progression of events. Study Design This study was part of a placebo-controlled double-blind clinical trial in which basal cell carcinomas were treated topically with an immunomodifier – toll-like receptor 7 agonist: imiquimod. The fourteen patients with basal cell carcinoma in the placebo arm of the trial received placebo treatment consisting solely of vehicle cream. A skin punch biopsy was obtained immediately before treatment and at the end of the placebo treatment (after 2, 4 or 8 days). 17.5K cDNA microarrays were utilized to profile the biopsy material. Results Four gene signatures whose expression changed relative to baseline (before wound induction by the pre-treatment biopsy) were identified. The largest group was comprised predominantly of inflammatory genes whose expression was increased throughout the study. Two additional signatures were observed which included preferentially pro-inflammatory genes in the early post-treatment biopsies (2 days after pre-treatment biopsies) and repair and angiogenesis genes in the later (4 to 8 days) biopsies. The fourth and smallest set of genes was down-regulated throughout the study. Early in wound healing the expression of markers of both M1 and M2 macrophages were increased, but later M2 markers predominated. Conclusion The initial response to a cutaneous wound induces powerful transcriptional activation of pro-inflammatory stimuli which may alert the host defense. Subsequently and in the absence of infection, inflammation subsides and it is replaced by angiogenesis and remodeling. Understanding this transition which may be driven by a change from a mixed macrophage population to predominately M2 macrophages, may help the interpretation of the cellular and molecular events occurring in the microenvironment of serially biopsied tissues. |
Databáze: | OpenAIRE |
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