Prolonged Adaptation to a Low or High Protein Diet Does Not Modulate Basal Muscle Protein Synthesis Rates - A Substudy
| Autor: | Margriet S. Westerterp-Plantenga, Rick Hursel, Henrike M. Hamer, Hanne K J Gonnissen, Luc J. C. van Loon, Joan M. G. Senden, Eveline A. P. Martens |
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| Přispěvatelé: | Humane Biologie, Nutrition and Movement Sciences, RS: NUTRIM - R3 - Chronic inflammatory disease and wasting, RS: NUTRIM - R1 - Metabolic Syndrome, RS: NUTRIM - HB/BW section A, RS: NUTRIM - HB/BW section B |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
030309 nutrition & dietetics medicine.medical_treatment Protein metabolism Muscle Proteins lcsh:Medicine High-protein diet medicine.disease_cause Basal (phylogenetics) chemistry.chemical_compound 0302 clinical medicine Protein biosynthesis Insulin AMINO-ACIDS lcsh:Science 2. Zero hunger 0303 health sciences Multidisciplinary MEN Fasting medicine.anatomical_structure BODY-WEIGHT SKELETAL-MUSCLE Female Dietary Proteins Research Article Adult medicine.medical_specialty Nitrogen Phenylalanine LEUCINE 030209 endocrinology & metabolism Carbohydrate metabolism Biology METABOLISM 03 medical and health sciences Young Adult Internal medicine medicine Diet Protein-Restricted Humans Muscle Skeletal KINETICS lcsh:R ENERGY-EXPENDITURE Skeletal muscle Metabolism Endocrinology Glucose PHYSICAL-ACTIVITY MAINTENANCE chemistry Protein Biosynthesis Tyrosine lcsh:Q |
| Zdroj: | PLOS ONE, 10(9):e0137183. Public Library of Science PLoS ONE, Vol 10, Iss 9, p e0137183 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0137183 |
| Popis: | BACKGROUND: Based on controlled 36 h experiments a higher dietary protein intake causes a positive protein balance and a negative fat balance. A positive net protein balance may support fat free mass accrual. However, few data are available on the impact of more prolonged changes in habitual protein intake on whole-body protein metabolism and basal muscle protein synthesis rates. OBJECTIVE: To assess changes in whole-body protein turnover and basal muscle protein synthesis rates following 12 weeks of adaptation to a low versus high dietary protein intake. METHODS: A randomized parallel study was performed in 40 subjects who followed either a high protein (2.4 g protein/kg/d) or low protein (0.4 g protein/kg/d) energy-balanced diet (30/35/35% or 5/60/35% energy from protein/carbohydrate/fat) for a period of 12 weeks. A subgroup of 7 men and 8 women (body mass index: 22.8+/-2.3 kg/m2, age: 24.3+/-4.9 y) were selected to evaluate the impact of prolonged adaptation to either a high or low protein intake on whole body protein metabolism and basal muscle protein synthesis rates. After the diet, subjects received continuous infusions with L-[ring-2H5]phenylalanine and L-[ring-2H2]tyrosine in an overnight fasted state, with blood samples and muscle biopsies being collected to assess post-absorptive whole-body protein turnover and muscle protein synthesis rates in vivo in humans. RESULTS: After 12 weeks of intervention, whole-body protein balance in the fasted state was more negative in the high protein treatment when compared with the low protein treatment (-4.1+/-0.5 vs -2.7+/-0.6 mumol phenylalanine/kg/h;P |
| Databáze: | OpenAIRE |
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