Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia: A Pilot Study
| Autor: | Michael Bachmann Nielsen, Kristoffer Lindskov Hansen, Stinne Byrholdt Sogaard, Sofie Bech Andersen, Jørgen Arendt Jensen, Lars Lönn, Charlotte Mehlin Sorensen, Carlos Armando Villagomez Hoyos, Fredrik Gran, Iman Taghavi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty kidney Super-resolution ultrasound imaging Clinical Biochemistry Ischemia microcirculation 030204 cardiovascular system & hematology Kidney Article Microcirculation 03 medical and health sciences 0302 clinical medicine SDG 3 - Good Health and Well-being Medicine super-resolution ultrasound imaging lcsh:R5-920 Renal ischemia rats Sprague-Dawley vasa recta reperfusion injury business.industry Ultrasound Blood flow medicine.disease Rats Reperfusion injury 030104 developmental biology medicine.anatomical_structure Vasa recta Microbubbles Renal vein business lcsh:Medicine (General) |
| Zdroj: | Diagnostics, Vol 10, Iss 862, p 862 (2020) Andersen, S B, Taghavi, I, Hoyos, C A V, Søgaard, S B, Gran, F, Lönn, L, Hansen, K L, Jensen, J A, Nielsen, M B & Sørensen, C M 2020, ' Super-Resolution Imaging with Ultrasound for Visualization of the Renal Microvasculature in Rats Before and After Renal Ischemia : A Pilot Study ', Diagnostics, vol. 10, no. 11, 862 . https://doi.org/10.3390/diagnostics10110862 Andersen, S B, Taghavi, I, Hoyos, C A V, Søgaard, S B, Gran, F, Lönn, L, Hansen, K L, Jensen, J A, Nielsen, M B & Sørensen, C M 2020, ' Super-resolution imaging with ultrasound for visualization of the renal microvasculature in rats before and after renal ischemia : A pilot study ', Diagnostics, vol. 10, no. 11, 862 . https://doi.org/10.3390/diagnostics10110862 Diagnostics Diagnostics; Volume 10; Issue 11; Pages: 862 |
| ISSN: | 2075-4418 |
| DOI: | 10.3390/diagnostics10110862 |
| Popis: | In vivo monitoring of the microvasculature is relevant since diseases such as diabetes, ischemia, or cancer cause microvascular impairment. Super-resolution ultrasound imaging allows in vivo examination of the microvasculature by detecting and tracking sparsely distributed intravascular microbubbles over a minute-long period. The ability to create detailed images of the renal vasculature of Sprague-Dawley rats using a modified clinical ultrasound platform was investigated in this study. Additionally, we hypothesized that early ischemic damage to the renal microcirculation could be visualized. After a baseline scan of the exposed kidney, 10 rats underwent clamping of the renal vein (n = 5) or artery (n = 5) for 45 min. The kidneys were rescanned at the onset of clamp release and after 60 min of reperfusion. Using a processing pipeline for tissue motion compensation and microbubble tracking, super-resolution images with a very high level of detail were constructed. Image filtration allowed further characterization of the vasculature by isolating specific vessels such as the ascending vasa recta with a 15–20 μm diameter. Using the super-resolution images alone, it was only possible for six assessors to consistently distinguish the healthy renal microvasculature from the microvasculature at the onset of vein clamp release. Future studies will aim at attaining quantitative estimations of alterations in the renal microvascular blood flow using super-resolution ultrasound imaging. |
| Databáze: | OpenAIRE |
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