Assessment of genetic diversity and bottleneck in Purnathadi buffaloes using short tandem repeat markers
| Autor: | Ramendra Das, Varinder Raina, Vikas Vohra, S.V. Kuralkar, S Sajid Ali, Ranjit S. Kataria |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
education.field_of_study Genetic diversity Buffaloes Population 0402 animal and dairy science Genetic Variation India Bioengineering 04 agricultural and veterinary sciences Biology 040201 dairy & animal science Bottleneck 03 medical and health sciences 030104 developmental biology Evolutionary biology parasitic diseases Microsatellite Animals Animal Science and Zoology education Biotechnology Microsatellite Repeats |
| Zdroj: | Animal biotechnology. 32(4) |
| ISSN: | 1532-2378 |
| Popis: | This study is the maiden attempt for genetic characterization of the Purnathadi, a phenotypically distinct buffalo population of the western Vidarbha region of Maharashtra and to explore genetic diversity using STR markers. A total of 48 unrelated Purnathadi buffaloes from the entire native tract were genotyped using a battery of 25 heterologous microsatellite markers. 5' end of forward primer of each microsatellite marker was labeled with one of the fluorescent dyes, viz., FAM (Blue), VIC (Green), NED (Yellow) or PET (Red) to assess the fragment length of genotyped PCR product with automated DNA sequencer (ABI 3100). 23 microsatellite loci (except ETH003 and ILSTS030) amplified successfully and adequately high allelic diversity (observed: 0.615 ± 0.043 and expected: 0.655 ± 0.037) was reported with 162 distinct microsatellite alleles. Sufficiently high Shannon index and PIC indicated the suitability of markers to evaluate genetic diversity in Purnathadi buffaloes. Within-population inbreeding estimates ( |
| Databáze: | OpenAIRE |
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