Reduced pathogenicity of aCandida albicansMAP kinase phosphatase (CPP1) mutant in the murine mastitis model
| Autor: | Henrik Jeldtoft Jensen, Csilla Csank, David Y. Thomas, Faisal A. Guhad, Malcolm Whiteway, Jann Hau |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
Microbiology (medical) Mutant Virulence Mastitis Protein tyrosine phosphatase Biology Pathology and Forensic Medicine Microbiology Mice Bacterial Proteins Candida albicans Animals pharmaceutical Immunology and Allergy Protein kinase A Gene Mice Inbred BALB C General Medicine biology.organism_classification Corpus albicans Mutation MAP kinase phosphatase Female Protein Tyrosine Phosphatases |
| Zdroj: | Europe PubMed Central University of Copenhagen |
| ISSN: | 1600-0463 0903-4641 |
| DOI: | 10.1111/j.1699-0463.1998.tb00257.x |
| Popis: | Candida albicans strains with a deletion of the mitogen-activated protein kinase tyrosine phosphatase gene (CPP1) are derepressed in the yeast-to-hyphal transition on solid surfaces in vitro at ambient temperatures and this gene is therefore required for repression of the yeast-to-hyphal switch. The pathology caused by a CPP1 null mutant strain was compared with that of the null mutant into which the wild-type CPP1 gene was introduced by homologous recombination and with the wild-type parent strain in a murine mycotic mastitis model. The mammary glands of lactating mice (at day 5 postpartum) were infected for 2, 4 and 6 days with 1 times 105, 1 times 106 and 1 times 107 cell-forming units before euthanasia. Infected and non-infected control glands were evaluated histopathologically. The null mutant strains showed less severe pathology than the two control strains. The Cpp1p tyrosine phosphatase may thus be considered a virulence determinant during localized infection in C. albicans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text