Immunization of Mice with a Live Transconjugant Shigella Hybrid Strain Induced Th1 and Th17 Cell-Mediated Immune Responses and Confirmed Passive Protection Against Heterologous Shigellae
| Autor: | Chaitali Sarkar, Hemanta Koley, Dhrubajyoti Nag, Jeffrey H. Withey, Priyadarshini Mukherjee, Ritam Sinha, Ratan Gachhui |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Shigella dysenteriae medicine.medical_treatment Immunology Administration Oral Heterologous Passive immunity Vaccines Attenuated medicine.disease_cause Shiga Toxin Microbiology Mice 03 medical and health sciences Immune system Species Specificity medicine Animals Shigella Dysentery Bacillary Antigens Bacterial Immunity Cellular Mice Inbred BALB C Virulence biology Immunization Passive Shiga toxin General Medicine Th1 Cells biology.organism_classification Antibodies Bacterial Bacterial vaccine Disease Models Animal 030104 developmental biology Animals Newborn Genes Bacterial Conjugation Genetic Bacterial Vaccines biology.protein Th17 Cells Female Antibody Gene Deletion |
| Zdroj: | Scandinavian Journal of Immunology. 83:92-101 |
| ISSN: | 0300-9475 |
| Popis: | An avirulent, live transconjugant Shigella hybrid (LTSHΔstx) strain was constructed in our earlier study by introducing a plasmid vector, pPR1347, into a Shiga toxin gene deleted Shigella dysenteriae 1. Three successive oral administrations of LTSHΔstx to female adult mice produced comprehensive passive heterologous protection in their offspring against challenge with wild-type shigellae. Production of NO and different cytokines such asIL-12p70, IL-1β and IL-23 in peritoneal mice macrophages indicated that LTSHΔstx induced innate and adaptive immunity in mice. Furthermore, production of IFN-γ, IL-10 and IL-17 in LTSH-primed splenic CD4+ T cell suggested that LTSHΔstx may induce Th1 and Th17 cell-mediated immune responses. Exponential increase of the serum IgG and IgA titre against whole shigellae was observed in immunized adult mice during and after the immunization with the highest peak on day 35. Antigen-specific sIgA was also determined from intestinal lavage of immunized mice. The stomach extracts of neonates from immunized mice, mainly containing mother's milk, contained significant levels of anti-LTSHΔstx immunoglobulin. These studies suggest that the LTSHΔstx could be a new live oral vaccine candidate against shigellosis in the near future. |
| Databáze: | OpenAIRE |
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