A twenty strain survey and backcross localization of the erythrocytic GTP concentration determining locus Gtpc on mouse chromosome 9
| Autor: | F F Snyder, F G Biddle, E Fung, G J Wiebe |
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| Rok vydání: | 1999 |
| Předmět: |
Genetic Markers
Erythrocytes GTP' Locus (genetics) Mice Inbred Strains Guanosine triphosphate Biology Kidney chemistry.chemical_compound Mice Adenosine Triphosphate Inbred strain Species Specificity Tongue Mice Inbred NOD Genetics Animals Nucleotide Molecular Biology Crosses Genetic chemistry.chemical_classification Mice Inbred C3H Kidney metabolism Brain Chromosome Mapping General Medicine Molecular biology Mice Inbred C57BL Muridae chemistry Biochemistry Liver Transferrin Guanosine Triphosphate Adenosine triphosphate Biotechnology Microsatellite Repeats |
| Zdroj: | Genome. 42(3) |
| ISSN: | 0831-2796 |
| Popis: | Erythrocyte nucleotide concentrations were surveyed among 20 inbred strains of mice in order to further assess the variability in GTP concentration. There was no significant difference in erythrocytic ATP concentration (Scheffé's test at P = 0.01), 678-1154 nmol/mL packed cells, among the strains surveyed. Two groups were distinguishable with respect to erythrocytic GTP concentration, 8 strains having high GTP, 215 ± 44 nmole/mL packed cells, and 12 strains having low GTP, 34 ± 12 nmole/mL packed cells. The erythrocytic GTP concentration determining trait Gtpc was previously shown to be linked to transferrin, Trf, on chromosome 9. Analysis of 232 [(B6 × WB) F1 × B6] backcross individuals for Gtpc and 8 microsatellite markers restricted the localization of Gtpc to a 5.6 ± 2.1 cM region. The gene order and genetic distances in cM ± SE are: (D9Mit14) 0.4 ± 0.4 (D9Mit24) 1.7 ± 0.8 (Gtpc, D9Mit51, D9Mit116, D9Mit212) 3.9 ± 1.3 (D9Mit200) 3.0 ± 1.1 (D9Mit20) 7.8 ± 1.8 (D9Mit18). The GTP concentration determining trait appears to be a property of erythrocytes as no differences were observed for GTP/ATP ratios of brain, kidney, liver, and tongue from a low GTP strain, C3H/HeHa × Pgk-1a and a high GTP strain, C57BL/6J.Key words: Gtpc, guanosine-5'-triphosphate, GTP, mouse, chromosome 9. |
| Databáze: | OpenAIRE |
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