Utilization of amino acids and dipeptides by Lactobacillus plantarum from orange in nutritionally stressed conditions
| Autor: | I.E. Loto Campos, F. M. Saguir, M. C. Manca de Nadra |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Citrus
amino acids and peptides Peptide Applied Microbiology and Biotechnology Beverages Ciencias Biológicas chemistry.chemical_compound Biología Celular Microbiología Animals Amino Acids Essential amino acid chemistry.chemical_classification Orange juice Bacteriological Techniques Dipeptide biology Dipeptides General Medicine Metabolism biology.organism_classification Culture Media Amino acid Meat Products Glucose nutrition chemistry Biochemistry Fermentation Food Microbiology Leucine metabolism CIENCIAS NATURALES Y EXACTAS Lactobacillus plantarum Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 104:1597-1604 |
| ISSN: | 1365-2672 1364-5072 |
| DOI: | 10.1111/j.1365-2672.2007.03708.x |
| Popis: | To investigate amino acid and dipeptide utilization by Lactobacillus plantarum N4 isolated from orange peel, in a nutritionally depleted medium basedon MRS (Mann, Rogosa, Sharpe).Methods and Results: In MRS with 0 Æ1 g l)1 of meat extract and without peptone and yeast extract, growth increased when essential and stimulatory amino acids and nonessential amino acid were added to the medium. Replacement of the essential amino acid, leucine, and the nonessential amino acid, glycine, byleucyl-leucine (Leu-Leu) and ⁄or glycyl-glycine (Gly-Gly) significantly enhancedgrowth. Essential amino acids were mainly consumed and the dipeptides were almost completely used at the end of growth. Leucine and glycine accumulatedinternally from the peptides were higher than from the free amino acids.Glucose utilization increased in the media containing dipeptides comparedwith the medium containing free amino acids.Conclusions: In a N-depleted medium, Leu-Leu and ⁄or Gly-Gly were more effective than the respective amino acids in supporting growth of the microorganism. The more efficient internal accumulation of glycine and especially leucine from dipeptides confirmed the ability of the strain to assimilate mainly complex nitrogen molecules rather than simple ones.Significance and Impact of the Study: The ability of Lact. plantarum N4 to efficiently use dipeptides could contribute to spoilage development in the natural medium of the organism, orange juice Fil: Saguir de Zucal, Fabiana Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina Fil: Loto Campos, Iris Eleonora. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina Fil: Manca de Nadra, M. C.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina |
| Databáze: | OpenAIRE |
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