Transthyretin synthesis in rabbit ciliary pigment epithelium
| Autor: | Keiichi Yamamoto, Yukio Ando, Hidenobu Tanihara, Masaaki Nakamura, Akiomi Takano, Akira Hirata, Takahiro Kawaji, Eiko Ando, Yasuya Inomata |
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| Rok vydání: | 2004 |
| Předmět: |
Lipopolysaccharides
Male endocrine system Pathology medicine.medical_specialty Amyloid Inflammation In situ hybridization Severity of Illness Index Uveitis Cellular and Molecular Neuroscience medicine Animals Prealbumin RNA Messenger Pigment Epithelium of Eye In Situ Hybridization Messenger RNA Retinal pigment epithelium biology Reverse Transcriptase Polymerase Chain Reaction Ciliary Body nutritional and metabolic diseases medicine.disease Molecular biology eye diseases Sensory Systems Epithelium Ophthalmology Transthyretin medicine.anatomical_structure Gene Expression Regulation biology.protein sense organs Rabbits medicine.symptom |
| Zdroj: | Experimental eye research. 81(3) |
| ISSN: | 0014-4835 |
| Popis: | Ocular symptoms of transthyretin (TTR)-related familial amyloidotic polyneuropathy (FAP) suggest that ciliary pigment epithelium (CPE) may synthesize TTR and its TTR may lead to amyloid formation in addition to TTR from vessels and retinal pigment epithelium (RPE). To clarify sites of TTR synthesis in ocular tissues, we performed in situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR) for qualitative detection of TTR mRNA. In addition, we quantified levels of TTR mRNA expression by means of real-time quantitative RT-PCR. Furthermore, although TTR is an anti-acute phase protein in serum level, no reports on changes in TTR expression in ocular tissues during acute inflammation exist. To investigate changes in TTR expression in ocular tissues during inflammation, we induced uveitis by endotoxin challenge in rabbits and used real-time quantitative RT-PCR to examine changes in TTR mRNA expression in ocular tissues. In situ hybridization and RT-PCR qualitatively demonstrated TTR mRNA not only in RPE but also in CPE. Real-time quantitative RT-PCR showed that the level of TTR mRNA expression in the CPE was about one-third of that in the RPE. TTR mRNA expression in ocular tissues decreased as the degree of inflammation increased. These results suggest that TTR synthesized in the CPE may lead to ocular manifestations, especially glaucoma, in FAP. TTR mRNA also acts as an anti-acute phase reactant in ocular tissues. |
| Databáze: | OpenAIRE |
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