Calcium accumulation and enzymatic activities of subcellular fractions from aortas and ventricles of genetically hypertensive rats
| Autor: | J W Wei, Edwin E. Daniel, R. A. Janis |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Male
medicine.medical_specialty Physiology Heart Ventricles chemistry.chemical_element Calcium Phosphodiesterase I Nucleotidases Internal medicine Nucleotidase medicine Animals Aorta chemistry.chemical_classification Phosphoric Diester Hydrolases Myocardium Cell Membrane Rats Inbred Strains Sucrose gradient Rats Enzyme Endocrinology chemistry Free calcium Hypertension Cardiology and Cardiovascular Medicine Marker enzymes Subcellular Fractions |
| Zdroj: | Circulation Research. 39:133-140 |
| ISSN: | 1524-4571 0009-7330 |
| DOI: | 10.1161/01.res.39.1.133 |
| Popis: | Subcellular fractions were obtained from aortas and ventricles of 6-month-old spontaneously hypertensive and normotensive Wistar rats by the use of differential and sucrose density gradient centrifugation. These preparations were studied to determine what alterations in calcium accumulation and enzymatic activities might be associated with hypertension. The total amount of calcium accumulation (in the presence of ATP and 17 muM free calcium) by the plasma membrane-enriched fraction from hypertensive rat aortas significantly less than that from normotensive rats (11.3 +/- 0.4 vs 16.2 +/- 1.6 mumol of calcium/g of protein, n = 8). In contrast the specific activities of the plasma membrane marker enzymes, 5'-nucleotidase and phosphodiesterase I, were 80% and 40% greater, respectively, in the hypertensive than in the normotensive fractions. On the other hand, various fractions from ventricles of the two types of rats were generally similar in enzyme activities and calcium accumulation. The decreased rate of relaxation of aortas from spontaneously hypertensive rats may be caused by the decreased rate of calcium transport demonstrated in this study. |
| Databáze: | OpenAIRE |
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