High-throughput fluorescence microscopic analysis of protein abundance and localization in budding yeast
| Autor: | Brandon Ho, Grant W. Brown, Nikko P. Torres |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Cell Saccharomyces cerevisiae Genomics Biology biology.organism_classification Biochemistry Fluorescence Protein subcellular localization prediction Yeast Cell biology High-Throughput Screening Assays 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Microscopy Fluorescence Proteome Fluorescence microscope medicine Molecular Biology |
| Zdroj: | Critical reviews in biochemistry and molecular biology. 51(2) |
| ISSN: | 1549-7798 |
| Popis: | Proteins directly carry out and regulate cellular functions. As a result, changes in protein levels within a cell directly influence cellular processes. Similarly, it is intuitive that the intracellular localization of proteins is a key component of their functionality. Optimal activity is achieved by a combination of protein concentration, co-compartmentalization with substrates, co-factors and regulators and sequestration from deleterious locales. The proteome within a cell is highly dynamic and changes in response to different environmental conditions. High-throughput microscopic analysis in the budding yeast Saccharomyces cerevisiae has afforded proteome-wide views of protein organization in living cells, and of how protein abundance and location is regulated and remodeled in response to stress. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |