B lymphocyte deficiency in IgH-transgenic rabbits
| Autor: | Katherine L. Knight, Pi Chen Yam, Susan L. Kalis, Periannan Sethupathi, Paul J. Jasper, Ki Jong Rhee, Shi Kang Zhai |
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| Rok vydání: | 2007 |
| Předmět: |
Genes
Immunoglobulin Heavy Chain Lymphocyte Immunology Naive B cell Fluorescent Antibody Technique Endogeny Biology Animals Genetically Modified Follicle medicine Animals Immunology and Allergy Amino Acid Sequence Receptor Immunity Mucosal B cell B-Lymphocytes Immunoglobulin mu-Chains Reverse Transcriptase Polymerase Chain Reaction Cell Differentiation Flow Cytometry Immunohistochemistry Molecular biology Epithelium Intestines medicine.anatomical_structure Immunoglobulin M Immunoglobulin G VDJ Exons Rabbits Bone marrow |
| Zdroj: | European Journal of Immunology. 37:2290-2299 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.200737191 |
| Popis: | We developed IgH-transgenic rabbits carrying a productive VDJ-Cmu Tg and found the rabbits were B cell-deficient, with a 50-100% reduction in serum IgM and IgG levels. The bone marrow of newborn Tg rabbits contained severely reduced levels of preB cells and almost no B cells. The few preB cells present in the bone marrow were large, cycling cells that expressed the VDJ-Cmu Tg, indicating that the block in B cell development likely occurred at or before the transition from large (early) preB to small (late) preB cells. By immunoprecipitation, the Tg mu-chain paired with VpreB and lambda5, suggesting that the B cell deficiency is not due to an inability to form a preB cell receptor. Despite the block in B cell development, a few B cells, expressing predominantly endogenous mu-chains, began the second stage of development in GALT. B cells were localized in and beneath the follicle-associated epithelium of GALT prior to B cell follicle formation, suggesting to us that B cell follicle formation is initiated near the follicle-associated epithelium, possibly through contact with intestinal microbiota. These IgH-Tg rabbits should provide a useful model for studies of B cell development both in bone marrow and in GALT. |
| Databáze: | OpenAIRE |
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