Using fluorescence to locate intercalants within the lipid bilayer of liposomes, bioliposomes and erythrocyte ghosts
| Autor: | Miriam E. Naqqash, Michal Afri, Aryeh A. Frimer |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Liposome
Chemistry Organic Chemistry Intercalation (chemistry) Cell Membrane Erythrocyte Membrane Lipid Bilayers Analytical chemistry Cell Biology Ligands Biochemistry Fluorescence Models Biological Solvent Cell membrane medicine.anatomical_structure Erythrocyte Ghosts Liposomes medicine Biophysics Solvents Emission spectrum Lipid bilayer Molecular Biology |
| Zdroj: | Chemistry and physics of lipids. 164(8) |
| ISSN: | 1873-2941 |
| Popis: | In previous work, we have shown the utility of the "NMR technique" in locating intercalants within the lipid bilayer. We describe herein the development of a more sensitive and complementary "fluorescence technique" for this purpose and its application to liposomes, bioliposomes and erythrocyte ghosts. This technique is based on the observation in selected compounds of an excellent correlation between the emission wavelength (λ(em)) and Dimroth-Reichardt E(T)(30) polarity parameter for the solvent in which the fluorescence emission spectrum was obtained. |
| Databáze: | OpenAIRE |
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