Nucleoplasmin facilitates reprogramming and in vivo development of bovine nuclear transfer embryos
| Autor: | Cena Myers, Jeffery M. Betthauser, Paul J. Golueke, Bing Liu, Ina Hoeschele, Gregory H. Leno, Hongzhi Xu, Kenneth J. Eilertsen, Jenine C. Lacson, Martha Pfister-Genskow, Richard W. Koppang |
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| Rok vydání: | 2006 |
| Předmět: |
Nuclear Transfer Techniques
Embryo Nonmammalian Somatic cell Cloning Organism Biology Chromatin remodeling Xenopus laevis Pregnancy Gene expression Genetics medicine Animals Blastocyst Nucleoplasmins Oligonucleotide Array Sequence Analysis Cell Nucleus Gene Expression Profiling Nuclear Proteins Cell Biology Chromatin Assembly and Disassembly Embryo Mammalian Phosphoproteins Embryonic stem cell Molecular biology Cell biology Chromatin medicine.anatomical_structure Polyglutamic Acid embryonic structures Somatic cell nuclear transfer Cattle Female Reprogramming Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 73:977-986 |
| ISSN: | 1098-2795 1040-452X |
| Popis: | Successful cloning by somatic cell nuclear transfer (NT) involves an oocyte-driven transition in gene expression from an inherited somatic pattern, to an embryonic form, during early development. This reprogramming of gene expression is thought to require the remodeling of somatic chromatin and as such, faulty and/or incomplete chromatin remodeling may contribute to the aberrant gene expression and abnormal development observed in NT embryos. We used a novel approach to supplement the oocyte with chromatin remodeling factors and determined the impact of these molecules on gene expression and development of bovine NT embryos. Nucleoplasmin (NPL) or polyglutamic acid (PGA) was injected into bovine oocytes at different concentrations, either before (pre-NT) or after (post-NT) NT. Pre-implantation embryos were then transferred to bovine recipients to assess in vivo development. Microinjection of remodeling factors resulted in apparent differences in the rate of blastocyst development and in pregnancy initiation rates in both NPL- and PGA-injected embryos, and these differences were dependent on factor concentration and/or the time of injection. Post-NT NPL-injected embryos that produced the highest rate of pregnancy also demonstrated differentially expressed genes relative to pre-NT NPL embryos and control NT embryos, both of which had lower pregnancy rates. Over 200 genes were upregulated following post-NT NPL injection. Several of these genes were previously shown to be downregulated in NT embryos when compared to bovine IVF embryos. These data suggest that addition of chromatin remodeling factors to the oocyte may improve development of NT embryos by facilitating reprogramming of the somatic nucleus. |
| Databáze: | OpenAIRE |
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