Antitumour activity of 3-chlorodeoxylapachol, a naphthoquinone from Avicennia germinans collected from an experimental plot in southern Florida
| Autor: | Geoffrey A. Cordell, Steven M. Swanson, A. Douglas Kinghorn, Tatiana Lobo-Echeverri, Qiuwen Mi, Djaja D. Soejarto, John M. Pezzuto, Dongho Lee, William P. Jones, Heebyung Chai |
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| Rok vydání: | 2005 |
| Předmět: |
Magnetic Resonance Spectroscopy
Pharmaceutical Science Antineoplastic Agents Fractionation Secondary metabolite Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Cell Line Tumor Botany medicine Humans Cytotoxic T cell Petroleum ether Cytotoxicity Chromatography High Pressure Liquid Pharmacology Geography Molecular Structure Plant Stems biology Plant Extracts Methanol Avicennia germinans Deuterium biology.organism_classification Naphthoquinone Plant Leaves chemistry Biochemistry Cell culture Florida Avicennia Drug Screening Assays Antitumor Naphthoquinones medicine.drug |
| Zdroj: | Journal of Pharmacy and Pharmacology. 57:1101-1108 |
| ISSN: | 2042-7158 0022-3573 |
| DOI: | 10.1211/jpp.57.9.0005 |
| Popis: | As part of an ongoing collaborative effort to discover new anticancer agents from plants, an extract obtained from the leaves and twigs of Avicennia germinans, collected in a coastal area of southern Florida, was identified as possessing cytotoxic activity in a panel of human cancer cell lines. Fractionation of the petroleum ether partition, using cytotoxicity to guide the fractionation, led to the isolation of 3-chlorodeoxylapachol. The antitumour potential of 3-chlorodeoxylapachol was demonstrated with the in-vivo hollow fibre assay, a model of antitumour activity using human cancer cell-filled fibres implanted into mice. The possibility that this compound is an artefact of the isolation procedure was ruled out by liquid chromatography—mass spectrometry analysis of extracts prepared without the use of chlorinated solvent. In conclusion, 3-chlordeoxylapachol, a secondary metabolite obtained from the chloroform-soluble extract of a mangrove tree, was cytotoxic in a panel of human cancer cells, and active against KB human cancer cells in the murine hollow fibre antitumour model, with selectivity in KB cells for the intravenous site at lower doses, indicating possible metabolic activation. |
| Databáze: | OpenAIRE |
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