A Bioinformatics Approach to the Identification of Variants Associated with Type 1 and Type 2 Diabetes Mellitus that Reside in Functionally Validated miRNAs Binding Sites
| Autor: | Hamid Ghaedi, Omar Ghaderi, Behnam Alipoor, Ziba Nariman-Saleh-Fam, Andrea Masotti, Reza Mirfakhraie, Mir Davood Omrani, Maryam Tabasinezhad, Abolfazl Movafagh, Mohammad Mehdi Jahani, Milad Bastami |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Linkage disequilibrium MiRNA binding Genome-wide association study Single-nucleotide polymorphism Biology Bioinformatics Biochemistry Polymorphism Single Nucleotide 03 medical and health sciences 0302 clinical medicine Databases Genetic Genetics Humans Genetic Predisposition to Disease RNA Messenger Molecular Biology Ecology Evolution Behavior and Systematics Genetic association Binding Sites Type 2 Diabetes Mellitus Computational Biology General Medicine Human genetics DNA binding site MicroRNAs 030104 developmental biology Diabetes Mellitus Type 1 Diabetes Mellitus Type 2 030220 oncology & carcinogenesis Genome-Wide Association Study |
| Zdroj: | Biochemical genetics. 54(3) |
| ISSN: | 1573-4927 |
| Popis: | The present work is aimed at finding variants associated with Type 1 and Type 2 diabetes mellitus (DM) that reside in functionally validated miRNAs binding sites and that can have a functional role in determining diabetes and related pathologies. Using bioinformatics analyses we obtained a database of validated polymorphic miRNA binding sites which has been intersected with genes related to DM or to variants associated and/or in linkage disequilibrium (LD) with it and is reported in genome-wide association studies (GWAS). The workflow we followed allowed us to find variants associated with DM that also reside in functional miRNA binding sites. These data have been demonstrated to have a functional role by impairing the functions of genes implicated in biological processes linked to DM. In conclusion, our work emphasized the importance of SNPs located in miRNA binding sites. The results discussed in this work may constitute the basis of further works aimed at finding functional candidates and variants affecting protein structure and function, transcription factor binding sites, and non-coding epigenetic variants, contributing to widen the knowledge about the pathogenesis of this important disease. |
| Databáze: | OpenAIRE |
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