Replication of tobacco ringspot virus

Autor: R.I.B. Francki, M.A. Rezaian
Rok vydání: 1974
Předmět:
Zdroj: Virology. 59:275-280
ISSN: 0042-6822
DOI: 10.1016/0042-6822(74)90223-2
Popis: Concentration of tobacco ringspot virus (TRSV) in cotyledons of cucumber and primary leaves of French bean increased linearly between 2 and 5 days after inoculation but decreased thereafter. The proportion of middle (M) to bottom (B) component in TRSV preparations from cucumber cotyledons did not vary significantly between 3 and 12 days after inoculation. High molecular weight double-stranded RNA (ds-RNA) with the expected properties of either replicative form or replicative intermediate was not detected in TRSV-infected plants. However, a polydisperse population of low molecular weight ds-RNA with nucleotide sequences complementary to TRSV-RNA was isolated. Although the function of this virus-specific ds-RNA remains obscure, it may be involved in the synthesis of TRSV. The TRSV-specific ds-RNA could be isolated in high yields only from leaves in which virus was increasing and its concentration in leaf tissues was positively correlated with the concentration of virus-induced RNA-dependent RNA polymerase.
Databáze: OpenAIRE