Biomechanical, Histological, and Molecular Characterization of a New Post-traumatic Model of Arthrofibrosis in Rats
| Autor: | Jacob W. Bettencourt, Aaron R. Owen, Daniel J. Berry, Afton K. Limberg, Joaquin Sanchez-Sotelo, Banu Bayram, Mark E. Morrey, Brad Bolon, Andre J. van Wijnen, Matthew P. Abdel, Louis Dagneaux |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Contracture
Knee Joint 0206 medical engineering Rat model 02 engineering and technology Article 03 medical and health sciences 0302 clinical medicine Fibrosis Medicine Animals Orthopedics and Sports Medicine Clinical significance Range of Motion Articular Arthroplasty Replacement Knee Arthrofibrosis 030203 arthritis & rheumatology business.industry Immobilization procedure Rodent model medicine.disease 020601 biomedical engineering Rats Rabbits medicine.symptom Joint Diseases business Nuclear medicine Complication |
| Zdroj: | J Orthop Res |
| Popis: | Experimental analyses of post-traumatic knee arthrofibrosis utilize a rabbit model as a gold standard. However, a rodent model of arthrofibrosis offers many advantages including reduced cost and comparison with other models of organ fibrosis. This study aimed to characterize the biomechanical, histological, and molecular features of a novel post-traumatic model of arthrofibrosis in rats. 48 rats were divided into two equal groups. An immobilization procedure was performed on the right hind limbs of experimental rats. One group was immobilized for 4 weeks and the other for 8 weeks. Both groups were remobilized for 4 weeks. Limbs were studied biomechanically via assessment of torque versus degree of extension, histologically via whole knee specimen, and molecularly via gene expression of posterior capsular tissues. Significant differences were observed between experimental and control limbs at 4 N-cm of torque in the 4-week (knee extension: 115° ± 8° vs. 169° ± 17°, respectively; p=0.007) and 8-week immobilization groups (knee extension: 99° ± 12° vs. 174° ± 9°, respectively; p=0.008). Histologically, in each group experimental limbs demonstrated increased posterior capsular thickness and total area of tissue when compared to control limbs (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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