High resolution MALDI-TOF-MS and MS/MS: Application for the structural characterization of sulfated oligosaccharides
| Autor: | José Kovensky, Gilbert Duhirwe, David Lesur |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Chromatography Molecular Structure Sulfates Chemistry 010401 analytical chemistry Oligosaccharides General Medicine Oligosaccharide Tandem mass spectrometry Mass spectrometry 01 natural sciences Atomic and Molecular Physics and Optics 0104 chemical sciences Matrix (chemical analysis) 03 medical and health sciences Matrix-assisted laser desorption/ionization Sulfation Tandem Mass Spectrometry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Desorption Tetrasaccharide Spectroscopy 030304 developmental biology |
| Zdroj: | European Journal of Mass Spectrometry. 25:428-436 |
| ISSN: | 1751-6838 1469-0667 |
| DOI: | 10.1177/1469066719851438 |
| Popis: | Sulfated oligosaccharides are involved in important biological events that are often modulated by specific sequences and sulfation patterns, but their structural analysis remains challenging. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) analysis of three different sulfated oligosaccharides (Fondaparinux, the octasulfated pentasaccharide, a disulfated heparin-derived tetrasaccharide 1, and an octasulfated maltoheptaose) 2 was performed using the 2-(4-hydroxyphenylazo)benzoic acid-tetramethylguanidinium (HABA-TMG2) matrix. High resolution mass spectrometry of the main ions observed was successful, and this was complemented by tandem mass spectrometry (MS/MS) analysis for structural assessment. Despite sulfate losses, fully sulfated molecular ions were observed and these allowed the determination of oligosaccharide structures: UA-GlcNAc-UA(2S)-AnhMan(6S) for compound 1 and (Glc6S)6-Glc (1S,6S) for compound 2. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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