Determination of urinary 6-mercaptopurine and three of its metabolites by HPLC–UV coupled with the iodine–azide reaction
| Autor: | Wojciech Młynarski, Joanna Trelińska, Adam Łuczak, Kamila Borowczyk, Robert Zakrzewski |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Azides
Time Factors Sodium Clinical Biochemistry chemistry.chemical_element Urine Urinalysis Iodine Analytical Chemistry chemistry.chemical_compound Limit of Detection medicine Humans General Pharmacology Toxicology and Pharmaceutics Child Acetonitrile Chromatography High Pressure Liquid Chromatography Mercaptopurine General Medicine Riboside Medical Laboratory Technology chemistry Sodium azide Spectrophotometry Ultraviolet Azide medicine.drug |
| Zdroj: | Bioanalysis. 5:869-877 |
| ISSN: | 1757-6199 1757-6180 |
| DOI: | 10.4155/bio.13.46 |
| Popis: | Background: The presented method is able to determine 6-mercaptopurine (6-MP), 6-thioguanine, 6-mercaptopurine riboside and 6-thioguanine riboside in urine, and is thereby dedicated to control of thiopurine therapy of children with acute lymphoblastic leukemia. Good separation of the mentioned compounds was achieved on a C18 stationary phase with a sodium azide and sodium heptane sulfonate solution, acetonitrile and water at ratio of 50:1:49 (v/v/v). Results: Coefficient of regression is >0.99 for all linearity ranges. LOD and LOQ are 0.3, 0.4, 0.3, 0.8 and 0.4, 0.6, 0.5 and 0.9 nmol/ml of urine for 6-MP, 6-thioguanine, 6-mercaptopurine riboside and 6-thioguanine riboside, respectively. Intra- and inter-day recovery and RSD are close to 100% and less than 10%, respectively, for all investigated thiopurines. Conclusion: The elaborated method was successfully applied for detection and quantitation of 6-MP and its selected metabolites in patients’ urine samples. |
| Databáze: | OpenAIRE |
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