Opsonic Activities of Surfactant Proteins A and D in Phagocytosis of Gram-Negative Bacteria by Alveolar Macrophages
| Autor: | J. C. Pikaar, W. F. Voorhout, L.M.G. Van Golde, Jan Verhoef, J. A. G. Van Strijp, J. F. Van Iwaarden |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Lipopolysaccharides
Male Pulmonary Surfactant-Associated Proteins Gram-negative bacteria Lipopolysaccharide Proteolipids Immunoelectron microscopy Phagocytosis Microbiology chemistry.chemical_compound Gram-Negative Bacteria Macrophages Alveolar Escherichia coli medicine Animals Immunology and Allergy Macrophage Rats Wistar Glycoproteins Pulmonary Surfactant-Associated Protein A biology Pulmonary Surfactants Macrophage Activation Opsonin Proteins respiratory system Pulmonary Surfactant-Associated Protein D biology.organism_classification Rats Infectious Diseases medicine.anatomical_structure chemistry Microscopy Electron Scanning Alveolar macrophage Binding Sites Antibody Pulmonary alveolus Bacterial outer membrane |
| Zdroj: | Journal of Infectious Diseases. 172:481-489 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/172.2.481 |
| Popis: | Surfactant proteins A and D (SP-A, SP-D) can interact with lipopolysaccharide (LPS) and stimulate alveolar macrophages. The opsonic activities of SP-A and SP-D for bacteria with different types of LPS and alveolar macrophages were investigated. In flow cytometric studies with fluorescein-labeled rough (J5) and smooth (O111) Escherichia coli and rat alveolar macrophages, SP-A enhanced binding of J5 but not O111 bacteria to macrophages. Most importantly, SP-A enhanced ingestion of J5 bacteria by alveolar macrophages and subsequent bacterial killing. Immunoelectron microscopy demonstrated that J5 bacteria, the interface between the bacterium and the outer membrane of the alveolar macrophage, and ingested bacteria were heavily labeled with SP-A. In contrast, SP-D did not mediate phagocytosis. SP-A acted as an opsonin in the phagocytosis of rough LPS-containing bacteria by alveolar macrophages, emphasizing the possible role for SP-A in the alveolar defense system. |
| Databáze: | OpenAIRE |
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