In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog
| Autor: | Konstantin Maslov, Alexei V. Demchenko, Archana R. Parameswar, Lihong V. Wang, Junjie Yao, Vassiliy Tsytsarev |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Fluorescence-lifetime imaging microscopy medicine.medical_specialty Convulsants Carbohydrate metabolism Pharmacology Deoxyglucose Article Rats Sprague-Dawley Epilepsy Internal medicine medicine Image Processing Computer-Assisted Animals 4-Aminopyridine Fluorescent Dyes Chemistry General Neuroscience Brain medicine.disease Fluorescence Rats Endocrinology 4-Chloro-7-nitrobenzofurazan Glucose Hypermetabolism Preclinical imaging medicine.drug |
| Popis: | Accurately locating epileptic foci has great importance in advancing the treatment of epilepsy. In this study, epileptic seizures were first induced by intracortical injection of 4-aminopyridine in rats. A fluorescent deoxyglucose substitute, 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG), was then continuously injected via the tail vein. Brain glucose metabolism was subsequently monitored by fluorescence imaging of 2-NBDG. The initial uptake rate of 2-NBDG at the injection site of 4-aminopyridine significantly exceeded that of the control injection site, which indicated local hypermetabolism induced by seizures. Our results show that 2-NBDG can be used for localizing epileptic foci. |
| Databáze: | OpenAIRE |
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