Anin vitromodel for assessment of the biological activity of hepatocyte growth factor
| Autor: | Carsten Peterson, Nasrin Perskvist, Kajsa Holmgren-Pettersson, Pia Forsberg, Tommy Sundqvist, Fariba Nayeri |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_treatment Clinical Biochemistry Motility Endogeny In Vitro Techniques Biology Models Biological Cell Line Mice Endocrinology Cell Movement In vivo Skin Ulcer medicine Animals Humans Aged Cell Proliferation Skin Wound Healing Hepatocyte Growth Factor Growth factor Epithelial Cells Biological activity Cell Biology Middle Aged Molecular biology Recombinant Proteins In vitro Cell culture Cancer research Female Hepatocyte growth factor medicine.drug |
| Zdroj: | Growth Factors. 25:33-40 |
| ISSN: | 1029-2292 0897-7194 |
| DOI: | 10.1080/08977190600997200 |
| Popis: | Hepatocyte growth factor (HGF) is a multifunctional growth factor with potent wound-healing properties that functions in the healing of chronic injuries. However, there may be a loss of HGF activity in certain chronic cases; this might be indicated by the presence of high amounts of HGF in body fluids and by the elevated expression of the HGF receptor in tissue biopsies. In such cases, a reliable means of assessing the activity of endogenous HGF would be valuable in allowing clinicians to decide if treatment with HGF would be useful. In this study, we developed an in vitro wound assay that used a mouse skin epithelial cell line to evaluate the biological activity of HGF. We showed that HGF accelerated the motility of the epithelial cells in a dose-dependent fashion with high sensitivity and specificity. This in vitro assay might be used to determine the activity of both endogenous and recombinant HGF. |
| Databáze: | OpenAIRE |
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