Fourier Transform Infrared Spectroscopic Imaging-Derived Collagen Content and Maturity Correlates with Stress in the Aortic Wall of Abdominal Aortic Aneurysm Patients
| Autor: | Joseph E. Pichamuthu, Mohammad F. Kiani, David A. Vorp, Rabee Cheheltani, Justin S. Weinbaum, Jayashree Rao, Nancy Pleshko |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Biomedical Engineering 030204 cardiovascular system & hematology Stress (mechanics) Extracellular matrix 03 medical and health sciences 0302 clinical medicine Degenerative disease Aneurysm medicine.artery Spectroscopy Fourier Transform Infrared medicine Humans Aorta Abdominal Aorta biology Chemistry Histology Anatomy medicine.disease Abdominal aortic aneurysm Elastin Extracellular Matrix 030104 developmental biology cardiovascular system biology.protein Collagen Stress Mechanical Cardiology and Cardiovascular Medicine Aortic Aneurysm Abdominal |
| Zdroj: | Cardiovascular engineering and technology. 8(1) |
| ISSN: | 1869-4098 |
| Popis: | Abdominal aortic aneurysm (AAA) is a degenerative disease of the aorta characterized by severe disruption of the structural integrity of the aortic wall and its major molecular constituents. From the early stages of disease, elastin in the aorta becomes highly degraded and is replaced by collagen. Questions persist as to the contribution of collagen content, quality and maturity to the potential for rupture. Here, using our recently developed Fourier transform infrared imaging spectroscopy (FT-IRIS) method, we quantified collagen content and maturity in the wall of AAA tissues in pairs of specimens with different wall stresses. CT scans of AAAs from 12 patients were used to create finite element models to estimate stress in different regions of tissue. Each patient underwent elective repair of the AAA, and two segments of the AAA tissues from anatomic regions more proximal or distal with different wall stresses were evaluated by histology and FT-IRIS after excision. For each patient, collagen content was generally greater in the tissue location with lower wall stress, which corresponded to the more distal anatomic regions. The wall stress/collagen ratio was greater in the higher stress region compared to the lower stress region (1.01 ± 1.09 vs. 0.55 ± 0.084, p = 0.02). The higher stress region also corresponded to the location with reduced intraluminal thrombus thickness. Further, collagen maturity tended to decrease with increased collagen content (p = 0.068, R = 0.38). Together, these results suggest that an increase in less mature collagen content in AAA patients does not effectively compensate for the loss of elastin in the aortic wall, and results in a reduced capability to endure wall stresses. |
| Databáze: | OpenAIRE |
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