Egg-hatching inhibition in mice immunized with recombinant Schistosoma bovis 28 kDa glutathione S-transferase
| Autor: | Jean-Marie Grzych, Sophia Lafitte, Josette Fontaine, Alexandra Viana da Costa, André Capron, Sophie Gaubert |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Immunology
Antibodies Helminth Heterologous Spleen Mice Immune system parasitic diseases medicine Animals Schistosomiasis Parasite Egg Count Glutathione Transferase Mice Inbred BALB C biology Interleukin Schistosoma mansoni biology.organism_classification Recombinant Proteins Schistosomiasis mansoni Glutathione S-transferase medicine.anatomical_structure Immunization biology.protein Cytokines Schistosoma Parasitology Female Antibody |
| Zdroj: | Parasite immunology. 21(7) |
| ISSN: | 0141-9838 |
| Popis: | The capacity of a recombinant glutathione S-transferase from Schistosoma bovis (rSb 28GST) to protect BALB/c mice against homologous and heterologous infections with, respectively, S. bovis or Schistosoma mansoni has been studied. Two injections of the rSb 28GST and an intravenous boost resulted in a marked specific IgG response on the day of experimental challenge with S. bovis or S. mansoni cercariae. Immunization of BALB/c mice led to a reduction in egg maturation and egg viability after infection with S. bovis or S. mansoni. Adult worm recoveries after an S. bovis challenge infection and tissue egg densities (intestine and liver) in S. mansoni challenge infection were also reduced in the immunized groups, but these differences were not statistically significant. No association between in vitro inhibition of GST enzymatic activity induced by immunized mouse sera and worm burden reduction was recorded. The analysis of the immune response, on the day of perfusion, showed the production of immunoglobulin (Ig)G1, IgG2a and IgG2b specific antibodies and the production of interleukin (IL)-4 and IL-5 by spleen cells after rSb 28GST stimulation. These data suggest that rSb 28GST immunization induces a moderate effect upon egg maturation and egg hatching, suggesting the involvement of similar mechanisms of action and common, but not exclusive, targets during S. bovis and S. mansoni infections. As a consequence, immunization with rSb 28GST may prove useful in affecting the pathology and transmission of African schistosomes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text