The characterization of a thermostable activator of β-d-glucosidase in normal human saliva
| Autor: | Rebecca Cherian, A.S. Balasubramanian |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
β d glucosidase
Saliva Hot Temperature Clinical Biochemistry Size-exclusion chromatography Biochemistry Chromatography Affinity Humans Polyacrylamide gel electrophoresis biology Activator (genetics) beta-Glucosidase Biochemistry (medical) General Medicine Molecular biology Enzyme Activation Sephadex Concanavalin A Pronase Chromatography Gel biology.protein Female Pronase digestion Glucosidases |
| Zdroj: | Clinica Chimica Acta. 110:169-176 |
| ISSN: | 0009-8981 |
| Popis: | The existence of thermostable activators of β- d -glucosidase (EC 3.2.1.21) in Gaucher tissues is known. We demonstrate the presence of a thermostable factor in normal human saliva which activates the β3- d -glucosidase of saliva. The activator is specific for β-glucosidase. It is dialysable and susceptible to pronase digestion. Sephadex G-25 gel filtration indicates that the activator is of low molecular weight, and polyacrylamide gel electrophoresis shows the low molecular weight fraction to migrate as two closely moving protein bands. A major proportion of the activator does not bind to concanavalin A-Sepharose. The activator appears to act by binding to or modifying the salivary β-glucosidase in a time-dependent manner. |
| Databáze: | OpenAIRE |
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