Femtosecond laser micro‐machined polyimide films for cell scaffold applications
| Autor: | Brigita Abakevičienė, Linas Šimatonis, Sigitas Tamulevičius, Aušra Gadeikytė, Ieva Antanavičiūtė, Vytenis Arvydas Skeberdis, Orestas Ulčinas, Tomas Tamulevičius, Edgaras Stankevičius, Valeryia Mikalayeva |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Trepanning Time Factors Materials science Finite Element Analysis Biomedical Engineering Medicine (miscellaneous) Young's modulus Cell Communication 02 engineering and technology Imides law.invention Biomaterials 03 medical and health sciences symbols.namesake law Elastic Modulus Tensile Strength Humans Quantum tunnelling Tissue Scaffolds business.industry Lasers Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Laser Biomechanical Phenomena Wavelength 030104 developmental biology Femtosecond symbols Microtechnology Optoelectronics 0210 nano-technology business Ultrashort pulse Polyimide |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 12 |
| ISSN: | 1932-7005 1932-6254 |
| Popis: | Engineering of sophisticated synthetic 3D scaffolds that allow controlling behavior and location of the cells requires advanced micro/nano fabrication techniques. Ultrafast laser micro-machining employing a 1030 nm wavelength Yb:KGW femtosecond laser and a micro-fabrication workstation for micro-machining of commercially available 12.7 and 25.4 µm thickness polyimide (PI) film was applied. Mechanical properties of the fabricated scaffolds, i.e., arrays of differently spaced holes, were examined via custom-built uniaxial micro-tensile testing and finite element method simulations. We demonstrate that experimental micro-tensile testing results could be numerically simulated and explained by 2-material model, assuming that 2-6 µm width rings around the holes possessed up to 5 times higher Young's modulus and yield stress compared with the rest of the laser intacted PI film areas of “dog-bone” shaped specimens. That was attributed to material modification around the micro-machined holes in the vicinity of the position of the focused laser beam track during trepanning drilling. We demonstrate that virgin PI films provide a suitable environment for the mobility, proliferation, and intercellular communication of human bone marrow mesenchymal stem cells and discuss how cell behavior varies on the micro-machined PI films with holes of different diameters (3.1, 8.4, and 16.7 µm) and hole spacing (30, 35, 40, and 45 µm). We conclude that the holes of 3.1 µm diameter were sufficient for metabolic and genetic communication through membranous tunneling tubes between cells residing on the opposite sides of PI film but prevented the trans-migration of cells through the holes. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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