Role of leukaemia inhibitory factor in the induction of pluripotent stem cells in mice
| Autor: | Feng Wang, Qiuyue Yan, Jun Wu, Zhouping Tang, Jie Xu, Xiong Yongjie, Suming Zhang, Jinping Zhang, Xuewei Xie, Yan-qiang Zhan |
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| Rok vydání: | 2010 |
| Předmět: |
Transition (genetics)
Somatic cell Induced Pluripotent Stem Cells Cell Differentiation Cell Biology General Medicine Biology Inhibitory postsynaptic potential Alkaline Phosphatase Cellular Reprogramming Molecular biology Leukemia Inhibitory Factor Induction system Cell biology Proto-Oncogene Proteins c-myc Mice embryonic structures Alkaline phosphatase Animals Induced pluripotent stem cell Leukemia inhibitory factor Reprogramming Signal Transduction |
| Zdroj: | Cell biology international. 34(8) |
| ISSN: | 1095-8355 |
| Popis: | iPS (induced pluripotent stem) cells can be induced from somatic cells in mice by genetic manipulation. Most previously established mouse iPS cell lines have been derived using feeder layers supplemented with exogenous LIF (leukaemia inhibitory factor). Although a feeder-free induction system has been developed in recent studies, LIF is still required for reprogramming, but its role in the generation of mouse iPS cells has remained elusive. In this study, we investigated its contribution to the induction of pluripotency. Our results showed that LIF activates AP (alkaline phosphatase) through a c-Myc-dependent mechanism. Moreover, it acts as a protective factor during the transition from AP-positive colonies to Oct3/4-positive cells. These findings illustrate a mechanism by which LIF may integrate signalling into reprogramming. |
| Databáze: | OpenAIRE |
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