Improvement of specificity of anti-testosterone and anti-5 alpha-dihydrotestosterone rabbit antibodies by immunotolerance techniques
| Autor: | M Rolland de Ravel, C. Grenot, J. Dupret, Elisabeth Mappus, Cuilleron Cy |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
endocrine system
medicine.medical_specialty Lysine Antigen-Antibody Complex Cross Reactions urologic and male genital diseases Biochemistry Antibodies Endocrinology Internal medicine polycyclic compounds medicine Immune Tolerance Animals Testosterone Bovine serum albumin Lagomorpha biology urogenital system Dihydrotestosterone Glutamic acid biology.organism_classification Immunization biology.protein Rabbits Antibody Hapten hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Journal of steroid biochemistry. 20(6A) |
| ISSN: | 0022-4731 |
| Popis: | Anti-testosterone (T) and anti-5 alpha-dihydrotestosterone (DHT) antibodies were raised in rabbits after preimmunization with 17 beta-hemisuccinamido, 7 beta-hemiglutaramido- and 3 beta-hemiglutaramido haptens of DHT and T covalently linked to D-glutamic acid-D-lysine (D-GL) copolymer, followed by immunization with the corresponding haptens covalently linked to bovine serum albumin. Preimmunization with DHT-D-GL in the case of anti-T-antibodies or with T-D-GL, in the case of anti-DHT antibodies significantly lowered the T-DHT cross-reactivity in all cases, the most striking effect being observed with anti-17 beta-hemisuccinamido-T antibodies (CR less than 1%). The evolution with time of the binding characteristics was also studied, showing that in several cases the lowered T-DHT cross-reactivity could be maintained after several booster injections until a useful titer was reached. The better results obtained with 17 beta-hemisuccinamido haptens suggest that the structure of the hapten exerts a strong influence on the induction of immunotolerance. |
| Databáze: | OpenAIRE |
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