Recombinant Ebola virus nucleoprotein and glycoprotein (Gabon 94 strain) provide new tools for the detection of human infections
| Autor: | V A Volchkova, Michèle Bouloy, Christophe Prehaud, D. Coudrier, E Hellebrand, Viktor E. Volchkov, Heinz Feldmann, B Le Guenno |
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| Rok vydání: | 1998 |
| Předmět: |
Genes
Viral viruses Molecular Sequence Data Enzyme-Linked Immunosorbent Assay Biology Antibodies Viral medicine.disease_cause Virus law.invention Viral Proteins law Virology medicine Humans Amino Acid Sequence Cloning Molecular Antigens Viral Escherichia coli Glycoproteins chemistry.chemical_classification Ebola virus Base Sequence Hemorrhagic Fever Ebola Ebolavirus Fusion protein Recombinant Proteins Nucleoprotein Nucleoproteins chemistry Immunoglobulin G Recombinant DNA biology.protein Antibody Glycoprotein |
| Zdroj: | Journal of General Virology. 79:2565-2572 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | After cloning and sequencing the glycoprotein (GP) gene of one of the Gabonese strains of Ebola virus isolated during the 1994-1996 outbreak, it was shown that the circulating virus was of the Zaire subtype. This was confirmed in this study by cloning and sequencing the nucleoprotein (NP) gene of this strain. These two structural proteins were also expressed as recombinant proteins and used in ELISA tests. NP was expressed as a His-tagged fusion protein in Escherichia coli and was purified on resins charged with nickel ions. GP was expressed by means of recombinant baculoviruses in Spodoptera frugiperda cells. Both recombinant proteins reacted positively in ELISAs for the detection of IgG antibodies in convalescent human sera from Gabon and Zaire. The difference in the relative titres of anti-NP and -GP antibodies was variable, depending on the sera. In addition, the recombinant NP reacted with heterologous sera from Côte d'Ivoire and was used successfully to detect IgM antibodies by mu-capture ELISA in sera from Gabonese patients. |
| Databáze: | OpenAIRE |
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