A microfluidic paper-based analytical device for the assay of albumin-corrected fructosamine values from whole blood samples
| Autor: | Yuwadee Boonyasit, Wanida Laiwattanapaisal |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Detection limit
Reproducibility Chromatography biology Chemistry Microfluidics Clinical Biochemistry Albumin Serum albumin Analytical chemistry General Medicine Microfluidic Analytical Techniques Analytical Chemistry Medical Laboratory Technology chemistry.chemical_compound Fructosamine Diabetes Mellitus biology.protein Humans General Pharmacology Toxicology and Pharmaceutics Serum Albumin Glycemic Whole blood |
| Zdroj: | Bioanalysis. 7:79-90 |
| ISSN: | 1757-6199 1757-6180 |
| Popis: | Background: A method for acquiring albumin-corrected fructosamine values from whole blood using a microfluidic paper-based analytical system that offers substantial improvement over previous methods is proposed. Results: The time required to quantify both serum albumin and fructosamine is shortened to 10 min with detection limits of 0.50 g dl-1 and 0.58 mM, respectively (S/N = 3). The proposed system also exhibited good within-run and run-to-run reproducibility. The results of the interference study revealed that the acceptable recoveries ranged from 95.1 to 106.2%. The system was compared with currently used large-scale methods (n = 15), and the results demonstrated good agreement among the techniques. Conclusion: The microfluidic paper-based system has the potential to continuously monitor glycemic levels in low resource settings. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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