Inhibitory effects of anions and active site amino acid sequence of chicken liver L-2-hydroxyacid oxidase A, a member of the FMN-dependent alpha-hydroxyacid oxidizing enzyme family
| Autor: | Liliane Dupuis, Antoine Puigserver, Josiane De Caro, Anne Ferjancic-Biagini |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Anions
Stereochemistry Proteolysis Molecular Sequence Data Biochemistry Oxalate chemistry.chemical_compound medicine Animals Dicarboxylic Acids Amino Acid Sequence Peptide sequence chemistry.chemical_classification Oxidase test Oxamic Acid Binding Sites biology medicine.diagnostic_test Sequence Homology Amino Acid Active site Substrate (chemistry) General Medicine Alcohol Oxidoreductases Kinetics Enzyme Dicarboxylic acid chemistry Liver biology.protein Chickens |
| Zdroj: | Biochimie. 80(12) |
| ISSN: | 0300-9084 |
| Popis: | Monocarboxylic acids with aliphatic chains were found to be mixed inhibitors of chicken liver L-2-hydroxyacid oxidase A when L-2-hydroxy-4-methylthiobutanoic acid was used as the substrate. The finding that the binding affinity of the enzyme for monocarboxylic acids was directly proportional to the number of carbon atoms in the chain strongly suggests that in addition to the electrostatic interaction due to the carboxyl moiety, hydrophobic forces may also be involved in the binding affinity of monocarboxylic acids to the enzyme's active site. Oxalate, a dicarboxylic acid, also resulted in a mixed-type inhibition of chicken liver L-2-hydroxyacid oxidase A, and, surprisingly, its binding affinity to the enzyme was found to be quite high as compared with monocarboxylic acids. This is probably due to the fact that the two carboxyl groups of oxalate give rise to electrostatic interactions with the positively charged side chains of two adjacent residues in the polypeptide chain. The inhibitory effects of other dicarboxylic acids was found to decrease as the number of carbon atoms in the chain increased. Oxamate was found however to be a novel type of potent inhibitor of the enzyme. All in all, these kinetic studies and the amino acid sequence determination in the active site region after limited proteolysis of the polypeptide chain definitely establish that chicken liver NADH/FMN containing L-2-hydroxyacid oxidase A is a member of the FMN-dependent α-hydroxyacid oxidizing enzyme family. |
| Databáze: | OpenAIRE |
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