Diosmetin protects against retinal injury via reduction of DNA damage and oxidative stress
| Autor: | Qiaojun He, Jiabin Dai, Jinjin Shao, Yuchen Lin, Jian Ma, Ke Yao, Zeren Shen, Xiaochun Yang, Peihua Luo, Bo Yang |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Chrysanthemum Health Toxicology and Mutagenesis Diosmetin (PubChem CID5281612) Apoptosis Toxicology medicine.disease_cause chemistry.chemical_compound 0302 clinical medicine Diosmetin Doxorubicin (PubChem CID31703) AMD age-related macular degeneration Genetics CNV choroidal neovascularisation Retinal injury IVI intravitreal injection humanities Cell biology medicine.anatomical_structure PVR proliferative vitreoretinopathy medicine.symptom IC50 inhibition for 50% of the cells Retinal Disorder ATP adenosine triphosphate RPE retinal pigment epithelium DNA damage Biology Article 03 medical and health sciences ROS reactive oxygen species lcsh:RA1190-1270 medicine DIO diosmetin Retinal pigment epithelium lcsh:Toxicology. Poisons Retina ADR adriamycin nutritional and metabolic diseases Retinal 030104 developmental biology Mechanism of action chemistry Oxidative stress 030221 ophthalmology & optometry H&E hematoxylin and eosin |
| Zdroj: | Toxicology Reports, Vol 3, Iss, Pp 78-86 (2016) Toxicology Reports Scipedia Open Access Scipedia SL |
| ISSN: | 2214-7500 |
| DOI: | 10.1016/j.toxrep.2015.12.004 |
| Popis: | Visual impairment is a global public health problem that needs new candidate drugs. Chrysanthemum is a traditional Chinese drug, famous for its eye-protective function, with an unclear mechanism of action. To determine how chrysanthemum contributes to vision, we identified, for the first time, the component of chrysanthemum, diosmetin (DIO), which acts in protecting the injured retina in an adriamycin (ADR) improving model. We observed that DIO could attenuate the apoptosis of retinal cells in Sprague–Dawley rats and verified this effect in cultured human retinal pigment epithelium (RPE) cells, ARPE-19. Our further study on the mechanism revealed the counteractive effect of DIO on the attenuation of DNA damage and oxidative stress, which occurs in a wide range of retinal disorders. These results collectively promise the potential value of DIO as a retinal-protective agent for disorders that lead to blindness. In addition, we identified, for the first time, the component of chrysanthemum, DIO, which acts in protecting the injured retina. Keywords: Apoptosis, Chrysanthemum, Diosmetin, DNA damage, Oxidative stress, Retinal injury, Retinal pigment epithelium |
| Databáze: | OpenAIRE |
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