Effects of peritoneal fluid from endometriosis patients on the release of monocyte-specific chemokines by leukocytes
| Autor: | Kyu-Sup Lee, Dong-Hyung Lee, Jong Kil Joo, Ji-Won Wang, Seungchul Kim, Jun-O Jin, Jong-Young Kwak, Y.J. Na |
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| Rok vydání: | 2010 |
| Předmět: |
CD4-Positive T-Lymphocytes
Chemokine Neutrophils T-Lymphocytes T cell Statistics as Topic Endometriosis Enzyme-Linked Immunosorbent Assay Stimulation CD8-Positive T-Lymphocytes Monocytes Leukocytes medicine Ascitic Fluid Humans Macrophage Chemokine CCL5 Cells Cultured Chemokine CCL2 Chemokine CCL3 biology business.industry Peritoneal fluid Monocyte Obstetrics and Gynecology Chemotaxis General Medicine medicine.anatomical_structure Cell culture Chemokines CC Immunology biology.protein Female business |
| Zdroj: | Archives of Gynecology and Obstetrics. 283:1333-1341 |
| ISSN: | 1432-0711 0932-0067 |
| Popis: | Chemokines have been implicated in the pathological process of endometriosis. We compared the effects of peritoneal fluid obtained from patients with endometriosis (ePF) and controls without endometriosis (cPF) on the release of monocyte-specific CC chemokines such as monocyte chemotactic protein-1 (MCP-1), regulated upon activation normal T cell expressed and secreted (RANTES), and macrophage inflammatory protein-1α (MIP-1α) by neutrophils, monocytes, and T cells. Moreover, we evaluated the correlation between the levels of chemokines in ePF and their release by these cells. Cells were obtained from healthy young volunteers and cultured with ePF (n = 12) or cPF (n = 8). The chemokine levels in the ePF and the supernatants of cultured cells with ePF were then measured by ELISA. There was a positive correlation between the levels of MCP-1 and MIP-1α in ePF. The addition of ePF to the cell cultures failed to increase the release of MCP-1, RANTES, and MIP-1α when compared to cPF, but the levels of RANTES in ePF were positively correlated with the release of RANTES by ePF-treated monocytes and T cells. Moreover, there was a positive correlation between the levels of RANTES and MIP-1α released by neutrophils and between the levels of MCP-1 and MIP-1α released by T cells. Finally, the levels of RANTES released by monocyte-derived macrophages and monocytes cultured with ePF were positively correlated. These findings suggest that monocytes, neutrophils, and T cells release differential levels of MCP-1, RANTES, and MIP-1α in response to stimulation with ePF. |
| Databáze: | OpenAIRE |
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