The biosynthesis of glucosamine
| Autor: | Carlos E. Cardini, Luis F. Leloir |
|---|---|
| Rok vydání: | 1953 |
| Předmět: |
Los Trabajos de Leloir
Cardini Carlos E Artículos Científicos Neurospora Neurospora crassa Los Trabajos del Instituto purl.org/becyt/ford/1 [https] Dephosphorylation chemistry.chemical_compound Biosynthesis Glucosamine Leloir Luis F purl.org/becyt/ford/1.6 [https] Leloir Investigador biology General Medicine biology.organism_classification Phosphate Glutamine Paper chromatography chemistry Biochemistry Publicaciones Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir |
| Zdroj: | Fundación Instituto Leloir. Archivo fondo LFL CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 0006-3002 |
| DOI: | 10.1016/0006-3002(53)90119-x |
| Popis: | A partially purified enzyme has been prepared from Neurospora crassa which catalyzes the formation of glucosamine phosphate from hexose-6-phosphate and glutamine. The glucosamine phosphate was identified by colour reactions, by dephosphorylation and paper chromatography and by its behaviour towards an acetylating system. Quantitative analysis of amide nitrogen, glutamate, and hexosamine agreed with the following equation: Hexose-6-phosphate + glutamine → glucosamine-6-phosphate + glutamate Crude Neurospora extracts were found to phosphorylate glucosamine in the presence of ATP and, when suitably supplemented, to acetylate glucosamine or glucosamine phosphate. |
| Databáze: | OpenAIRE |
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