Timing urinary tract reconstruction in rats to avoid hydronephrosis and fibrosis in the transplanted fetal metanephros as assessed using imaging
| Autor: | Satomi Iwai, Takafumi Haji, Takuya Matsumoto, Shozo Okano, Takashi Yokoo, Kenichi Maeda, Chisato Hayakawa, Kotaro Nishi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male 030232 urology & nephrology Transplants Hydronephrosis Kidney Diagnostic Radiology 0302 clinical medicine Pregnancy Ultrasound Imaging Medicine and Health Sciences Renal Transplantation Urinary Tract Tomography Kidney transplantation Multidisciplinary Radiology and Imaging Anastomosis Surgical medicine.anatomical_structure Medicine Female Anatomy Research Article medicine.medical_specialty Imaging Techniques Urinary system Science Bladder Urology Surgical and Invasive Medical Procedures Neuroimaging Research and Analysis Methods Urinary System Procedures 03 medical and health sciences Ureter Diagnostic Medicine Metanephros medicine Animals Regeneration Transplantation business.industry Biology and Life Sciences Kidneys Renal System Organ Transplantation medicine.disease Fibrosis Kidney Transplantation Rats Computed Axial Tomography 030104 developmental biology Rats Inbred Lew business Kidney disease Developmental Biology Neuroscience |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 1, p e0231233 (2021) |
| ISSN: | 1932-6203 |
| Popis: | Chronic kidney disease leads to high morbidity rates among humans. Kidney transplantation is often necessary for severe symptoms; however, options for new curative treatments are desired because of donor shortage. For example, it has been established that the kidneys can efficiently generate urine after transplantation of the metanephros, ureter, and bladder as a group. After transplantation, the urine can indirectly flow into the recipient’s bladder using a stepwise peristaltic ureter system method where the anastomosis is created via the recipient’s ureter for urinary tract reconstruction. However, the growth of the regenerated metanephros varies significantly, whereas the time window for successful completion of the stepwise peristaltic ureter system that does not cause hydronephrosis of the metanephros with bladder (ureter) is quite narrow. Therefore, this study was conducted to periodically and noninvasively evaluate the growth of the transplanted metanephros, ureter, and bladder in rats through computed tomography and ultrasonography. The ultrasonographic findings highly correlated to the computed tomography findings and clearly showed the metanephros and bladder. We found that the degree of growth of the metanephros and the bladder after transplantation differed in each case. Most of the rats were ready for urinary tract reconstruction within 21 days after transplantation. Optimizing the urinary tract reconstruction using ultrasonography allowed for interventions to reduce long-term tubular dilation of the metanephros due to inhibited overdilation of the fetal bladder, thereby decreasing the fibrosis caused possibly by transforming growth factor-β1. These results may be significantly related to the long-term maturation of the fetal metanephros and can provide new insights into the physiology of transplant regeneration of the metanephros in higher animals. Thus, this study contributes to the evidence base for the possibility of kidney regeneration in human clinical trials. |
| Databáze: | OpenAIRE |
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